Melnichenko N G, Zverinsky I V, Artsukevich I M, Lukienko P I
Laboratory of Biochemical Pharmacology, Institute of Biochemistry, National Academy of Sciences, Grodno, Belarus.
Exp Toxicol Pathol. 1999 Jul;51(4-5):389-91. doi: 10.1016/S0940-2993(99)80026-8.
The horse-radish peroxidase catalyzes oxidation of thiamine in vitro in presence of hydroperoxide hydrogen (H2O2) and olenic acid (LOOH) (as an emultion) with formation of thiochrome (2,7-dimethyl-8-(oxyetyl-5,6-dihydrothiazolo (2,3-a) pyrimido (4,5-d) pyrimidine, oxodihydrothiochrome (2,7dimethyl-oxo-8-(-2-oxo-ethyl)-5,6,9a, 10-tetrahydrothiazolo (2,3-a) pyrimido (4,5-d) pyrimidine and thiamine disulfide (bis (3-methyl-((1-methyl-4-amino 5 pyrimidine methylformyl) amino 1-2 (oxyethyl)-1-propenyl) disulfide. The transformation of thiamine to thiochrome cause by release of two atoms of hydrogen from NH2-group of pyrimidine ring of molecule of thiamine and occurrence on this place of bond with thiozole ring. The microsomal fraction of rats in vitro in presence of LOOH also activities the oxidation of thiamine to thiochrome. However in these conditions peroxidase reaction is proceeded more slowly.
在体外，辣根过氧化物酶在过氧化氢（H2O2）和油酸（LOOH，以乳液形式存在）存在的情况下催化硫胺素的氧化，生成硫色素（2,7 - 二甲基 - 8 -（氧乙基）- 5,6 - 二氢噻唑并（2,3 - a）嘧啶并（4,5 - d）嘧啶）、氧化二氢硫色素（2,7 - 二甲基 - 氧代 - 8 -（- 2 - 氧代 - 乙基）- 5,6,9a,10 - 四氢噻唑并（2,3 - a）嘧啶并（4,5 - d）嘧啶）和硫胺素二硫化物（双（3 - 甲基 -（（1 - 甲基 - 4 - 氨基 - 5 - 嘧啶甲基甲酰基）氨基 - 1 - 2 -（氧乙基）- 1 - 丙烯基）二硫化物）。硫胺素向硫色素的转化是由于硫胺素分子嘧啶环的NH2基团释放出两个氢原子，并在该位置与噻唑环形成键。在体外，大鼠微粒体部分在LOOH存在的情况下也能将硫胺素氧化为硫色素。然而，在这些条件下，过氧化物酶反应进行得更慢。
