Rev蛋白能够将含有Rev结合元件的小核仁RNA转运至细胞质。
The Rev protein is able to transport to the cytoplasm small nucleolar RNAs containing a Rev binding element.
作者信息
Buonomo S B, Michienzi A, De Angelis F G, Bozzoni I
机构信息
Istituto Pasteur, Fondazione Cenci-Bolognetti, Dipartimento di Genetica e Biologia Molecolare, Università di Roma La Sapienza, Italy.
出版信息
RNA. 1999 Aug;5(8):993-1002. doi: 10.1017/s1355838299990064.
Abstract
Small nucleolar RNAs (snoRNAs) were utilized to express Rev-binding sequences inside the nucleolus and to test whether they are substrates for Rev binding and transport. We show that U16 snoRNA containing the minimal binding site for Rev stably accumulates inside the nucleolus maintaining the interaction with the basic C/D snoRNA-specific factors. Upon Rev expression, the chimeric RNA is exported to the cytoplasm, where it remains bound to Rev in a particle devoid of snoRNP-specific factors. These data indicate that Rev can elicit the functions of RNA binding and transport inside the nucleolus.
摘要
小核仁RNA（snoRNAs）被用于在核仁内表达与Rev结合的序列，并测试它们是否是Rev结合和转运的底物。我们发现，含有Rev最小结合位点的U16 snoRNA在核仁内稳定积累，并与基本的C/D snoRNA特异性因子保持相互作用。Rev表达后，嵌合RNA被转运到细胞质中，在那里它仍然与Rev结合，存在于缺乏snoRNP特异性因子的颗粒中。这些数据表明，Rev可以在核仁内引发RNA结合和转运的功能。
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