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Nucleic Acid Storage in the Toad's Egg.蟾蜍卵中的核酸储存
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Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.对于前体rRNA的位点特异性核糖甲基化至关重要的甲基化指导snoRNA的序列和结构元件。
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A 7-methylguanosine cap commits U3 and U8 small nuclear RNAs to the nucleolar localization pathway.7-甲基鸟苷帽使U3和U8小核RNA进入核仁定位途径。
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Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits.剪接因子3b（SF3b）亚基的酵母同源物中结构和亚基相互作用的保守性。
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A small nucleolar RNP protein is required for pseudouridylation of eukaryotic ribosomal RNAs.真核生物核糖体RNA假尿嘧啶化需要一种小核仁核糖核蛋白。
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U14 small nucleolar RNA makes multiple contacts with the pre-ribosomal RNA.U14小核仁RNA与核糖体前体RNA有多处接触。
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Delocalization of some small nucleolar RNPs after actinomycin D treatment to deplete early pre-rRNAs.放线菌素D处理后一些小核仁核糖核蛋白的去定位以耗尽早期前体核糖体RNA。
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保守的C盒和D盒是U14和U8小核仁RNA的重要核仁定位元件。

Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.

作者信息

Lange T S, Borovjagin A, Maxwell E S, Gerbi S A

机构信息

Division of Biology and Medicine, Brown University, Providence, RI 02912, USA.

出版信息

EMBO J. 1998 Jun 1;17(11):3176-87. doi: 10.1093/emboj/17.11.3176.

DOI:10.1093/emboj/17.11.3176
PMID:9606199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1170656/
Abstract

Sequences necessary for nucleolar targeting were identified in Box C/D small nucleolar RNAs (snoRNAs) by fluorescence microscopy. Nucleolar preparations were examined after injecting fluorescein-labelled wild-type and mutated U14 or U8 snoRNA into Xenopus oocyte nuclei. Regions in U14 snoRNA that are complementary to 18S rRNA and necessary for rRNA processing and methylation are not required for nucleolar localization. Truncated U14 molecules containing Boxes C and D with or without the terminal stem localized efficiently. Nucleolar localization was abolished upon mutating just one or two nucleotides within Boxes C and D. Moreover, the spatial position of Boxes C or D in the molecule is essential. Mutations in Box C/D of U8 snoRNA also impaired nucleolar localization, suggesting the general importance of Boxes C and D as nucleolar localization sequences for Box C/D snoRNAs. U14 snoRNA is shown to be required for 18S rRNA production in vertebrates.

摘要

通过荧光显微镜在C/D盒小核仁RNA（snoRNAs）中鉴定出了核仁靶向所需的序列。将荧光素标记的野生型和突变型U14或U8 snoRNA注入非洲爪蟾卵母细胞核后，对核仁制剂进行了检查。U14 snoRNA中与18S rRNA互补且对rRNA加工和甲基化必需的区域，并非核仁定位所必需。含有C盒和D盒且带有或不带有末端茎的截短U14分子能高效定位。仅在C盒和D盒内突变一两个核苷酸就会消除核仁定位。此外，C盒或D盒在分子中的空间位置至关重要。U8 snoRNA的C/D盒突变也会损害核仁定位，这表明C盒和D盒作为C/D盒snoRNAs的核仁定位序列具有普遍重要性。已证明U14 snoRNA是脊椎动物中18S rRNA产生所必需的。