Schilling M K, Redaelli C, Friess H, Blum B, Signer C, Maurer C A, Büchler M W
Department of Visceral and Transplant Surgery, University of Bern, Inselspital, Bern, Switzerland.
Gut. 1999 Sep;45(3):341-5. doi: 10.1136/gut.45.3.341.
Tumour vascularisation is a determinant of the development of metastases.
To measure blood flow in normal stomach and gastric adenocarcinomas by laser Doppler flowmetry and correlate blood flow with vascularisation after immunohistochemical staining of resected specimens for CD31 and von Willebrand factor.
Twenty two undergoing resection for gastric adenocarcinoma and 10 undergoing cholecystectomy.
Mean (SD) gastric blood flow was 208 (35) perfusion units (PU) in patients undergoing cholecystectomy and 190 (75) PU in the undiseased part of the stomach in patients with gastric adenocarcinoma. Gastric blood flow was higher in the border of gastric adenocarcinomas (322 (120) PU, p<0.01 v normal stomach) but lower in the centre (74 (27) PU, p<0.01 v normal stomach and tumour border). Blood flow was higher in tumours staged T>/=3 than in those staged T<3. Blood vessel density in normal stomach was 41 (8) stained cells/field viewed and was 1. 9-3.4 times higher in gastric adenocarcinomas.
Laser Doppler flowmetry is a valuable tool for studying the pathophysiological alterations of malignant blood flow in the human stomach in vivo.
肿瘤血管形成是转移发生的一个决定因素。
通过激光多普勒血流仪测量正常胃组织和胃腺癌组织中的血流,并在对切除标本进行CD31和血管性血友病因子免疫组化染色后,将血流与血管形成情况进行关联分析。
22例行胃腺癌切除术的患者和10例行胆囊切除术的患者。
行胆囊切除术患者的平均(标准差)胃血流量为208(35)灌注单位(PU),胃腺癌患者未患病胃组织部分的平均胃血流量为190(75)PU。胃腺癌边界处的胃血流量较高(322(120)PU,与正常胃组织相比p<0.01),但中心处较低(74(27)PU,与正常胃组织和肿瘤边界相比p<0.01)。T≥3期肿瘤的血流量高于T<3期肿瘤。正常胃组织的血管密度为41(8)个染色细胞/视野,胃腺癌的血管密度比正常胃组织高1.9 - 3.4倍。
激光多普勒血流仪是在体研究人胃恶性血流病理生理改变的一种有价值的工具。