Iwao T, Toyonaga A, Ikegami M, Oho K, Sumino M, Sakaki M, Harada H, Tanikawa K
Second Department of Medicine, Kurume University School of Medicine, Japan.
Endoscopy. 1993 May;25(4):274-7. doi: 10.1055/s-2007-1010313.
We evaluated the reliability (i.e. reproducibility and variability) in measuring regional gastric mucosal blood flow by laser Doppler flowmetry in a total of 32 subjects (12 normal subjects and 20 patients with cirrhosis). The measurements were performed in quadruplicate within 10 min by observer A (A1, A2) and B (B1, B2) using blind techniques. The intraclass correlation coefficient (Ri) was used to assess observer agreement. Overall, the level of intra- (Ri, 0.87 for A1 vs. A2 and 0.87 for B1 vs. B2) and interobserver agreement (0.86 for A vs. B) was excellent. In contrast, there was a higher observer variability assessed by coefficient of variation. Overall, the mean value of coefficient of variation was 12% (max. 35%) for A1 vs. A2, 12% (max. 35%) for B1 vs. B2, and 13% (max. 33%) for A vs. B. These results suggest that laser Doppler flowmetry, although the reproducibility may be clinically acceptable, seems to still be a subjective method for quantitation of gastric mucosal blood flow in humans. Therefore, studies using this method can only be acceptable when the changes that investigators intend to measure are significantly higher than the variability.
我们评估了激光多普勒血流仪测量32名受试者（12名正常受试者和20名肝硬化患者）局部胃黏膜血流的可靠性（即可重复性和变异性）。测量由观察者A（A1、A2）和B（B1、B2）采用盲法技术在10分钟内重复进行4次。组内相关系数（Ri）用于评估观察者间的一致性。总体而言，组内一致性（A1与A2的Ri为0.87，B1与B2的Ri为0.87）和观察者间一致性（A与B的为0.86）非常好。相比之下，通过变异系数评估的观察者变异性较高。总体而言，A1与A2的变异系数平均值为12%（最大值35%），B1与B2的为12%（最大值35%），A与B的为13%（最大值33%）。这些结果表明，激光多普勒血流仪虽然其可重复性在临床上可能是可接受的，但似乎仍然是一种用于定量人体胃黏膜血流的主观方法。因此，只有当研究者想要测量的变化显著高于变异性时，使用该方法的研究才是可接受的。
