一种新型定量多重核酸序列扩增法：应用于检测人类单核细胞中组织因子和CD14 mRNA水平
A novel quantitative multiplex NASBA method: application to measuring tissue factor and CD14 mRNA levels in human monocytes.
作者信息
van Deursen P B, Gunther A W, van Riel C C, van der Eijnden M M, Vos H L, van Gemen B, van Strijp D A, Tackent N M, Bertina R M
机构信息
Organon Teknika B.V., Boseind 15, PO Box 84, 5280 AB Boxtel, The Netherlands.
出版信息
Nucleic Acids Res. 1999 Sep 1;27(17):e15. doi: 10.1093/nar/27.17.e15.
A new method to quantify two individual mRNAs in a single NASBA reaction is described. In this study, tissue factor and CD14 mRNAs were used as a model system. RNA ratios of -4 to +4 log units were determined with good precision (within 0.3 log) and accuracy (within 0.2 log). By measuring both mRNAs in human monocytes that were stimulated with LPS, the multiplex Q-NASBA proved to be a successful tool to monitor the expression levels of two individual mRNAs in a single-tube amplification system. The method has potential in all fields in which quantitative information is needed on two individual RNAs.
本文描述了一种在单个核酸序列扩增技术（NASBA）反应中对两种个体信使核糖核酸（mRNA）进行定量的新方法。在本研究中，组织因子和CD14的mRNA被用作模型系统。RNA比率在-4至+4对数单位之间测定，具有良好的精密度（在0.3对数范围内）和准确度（在0.2对数范围内）。通过测量经脂多糖刺激的人类单核细胞中的两种mRNA，多重定量NASBA被证明是在单管扩增系统中监测两种个体mRNA表达水平的成功工具。该方法在所有需要两种个体RNA定量信息的领域都具有潜力。
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