基于核酸序列的血液样本中曲霉菌RNA扩增
Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples.
作者信息
Loeffler J, Hebart H, Cox P, Flues N, Schumacher U, Einsele H
机构信息
Medizinische Klinik, Abteilung II, Eberhard-Karls-Universität Tübingen, 72076 Tübingen, Germany.
出版信息
J Clin Microbiol. 2001 Apr;39(4):1626-9. doi: 10.1128/JCM.39.4.1626-1629.2001.
Abstract
Nucleic acid sequence-based amplification (NASBA), an isothermal amplification technique, was established and evaluated for the detection of Aspergillus RNA and compared with a previously published, well-defined real-time PCR assay amplifying a region of the Aspergillus 18S rRNA gene. NASBA showed a lower detection limit of 1 CFU and detected RNA from five different clinically relevant Aspergillus species, including Aspergillus fumigatus. All 77 blood samples tested by PCR and NASBA showed identical results in both assays. Results with the NASBA technique were obtained within 6 h. Thus, the NASBA technique provided a valuable tool for sensitive, specific, fast, and reliable detection of Aspergillus RNA with potential for routine diagnosis, including the possibility to test the viability of cells.
摘要
基于核酸序列的扩增技术（NASBA）是一种等温扩增技术，已被建立并用于检测曲霉RNA，并与之前发表的、明确的实时PCR检测方法进行了比较，后者扩增曲霉18S rRNA基因的一个区域。NASBA的检测下限为1 CFU，能检测来自五种不同临床相关曲霉菌种的RNA，包括烟曲霉。通过PCR和NASBA检测的所有77份血样在两种检测方法中结果均相同。使用NASBA技术在6小时内即可获得结果。因此，NASBA技术为敏感、特异、快速且可靠地检测曲霉RNA提供了一种有价值的工具，具有常规诊断的潜力，包括检测细胞活力的可能性。
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