Gorbenko G P
Department of Physics and Technology, Kharkov State University, Svoboda Sq. 4, Kharkov 310077, Ukraine.
Biochim Biophys Acta. 1999 Aug 20;1420(1-2):1-13. doi: 10.1016/s0005-2736(99)00082-6.
Resonance energy transfer between a series of lipid-bound fluorescent probes as donors and the heme group of cytochrome c as acceptor has been used to obtain structural information on the protein complexes with model membranes, composed of phosphatidylcholine and cardiolipin. Analysis of experimental data in terms of the model of energy transfer in two-dimensional systems provides further evidence for preferential cytochrome c orientation with respect to the lipid bilayer and penetration of the protein into the membrane interior.
一系列与脂质结合的荧光探针作为供体，细胞色素c的血红素基团作为受体之间的共振能量转移，已被用于获取蛋白质与由磷脂酰胆碱和心磷脂组成的模型膜复合物的结构信息。根据二维系统中的能量转移模型对实验数据进行分析，为细胞色素c相对于脂质双层的优先取向以及蛋白质向膜内部的渗透提供了进一步的证据。
