Gorbenko G P
Department of Physics and Technology, Kharkov State University, Tobolskaya Str. 52-52, 310077 Kharkov, Ukraine.
Biochim Biophys Acta. 1998 Nov 2;1409(1):12-24. doi: 10.1016/s0005-2728(98)00140-6.
The complexes of hemoglobin and cytochrome c with liposomes composed of phosphatidylcholine and its mixtures with cardiolipin and cholesterol have been studied by monitoring resonance energy transfer between fluorescent probe 3-methoxybenzanthrone as donor and heme groups of the proteins as acceptors. By analyzing experimental data within the framework of the model of energy transfer in two-dimensional systems, the limits of the range of possible heme positions with respect to lipid bilayer have been assessed. The distance of heme group of hemoglobin from the membrane center was found to increase in the presence of cardiolipin or cholesterol. The results obtained for cytochrome c complexes with cardiolipin-containing model membranes suggest the existence of preferential protein orientation relative to the lipid bilayer, and provide evidence for the protein penetration in the membrane interior.
通过监测荧光探针3-甲氧基苯并蒽酮作为供体与蛋白质血红素基团作为受体之间的共振能量转移，研究了血红蛋白和细胞色素c与由磷脂酰胆碱及其与心磷脂和胆固醇的混合物组成的脂质体的复合物。通过在二维系统能量转移模型框架内分析实验数据，评估了血红素相对于脂质双层可能位置范围的极限。发现存在心磷脂或胆固醇时，血红蛋白血红素基团距膜中心的距离会增加。细胞色素c与含心磷脂模型膜复合物的研究结果表明，蛋白质相对于脂质双层存在优先取向，并为蛋白质渗透到膜内部提供了证据。
