抗菌肽乳铁蛋白B对多种抗生素作用于大肠杆菌和金黄色葡萄球菌的干扰。

Interference of the antimicrobial peptide lactoferricin B with the action of various antibiotics against Escherichia coli and Staphylococcus aureus.

作者信息

Vorland L H, Osbakk S A, Perstølen T, Ulvatne H, Rekdal O, Svendsen J S, Gutteberg T J

机构信息

Department of Medical Microbiology, University Hospital, Tromsø, Norway.

出版信息

Scand J Infect Dis. 1999;31(2):173-7. doi: 10.1080/003655499750006236.

DOI:10.1080/003655499750006236

PMID:10447328

Abstract

The antimicrobial peptide, lactoferricin, can be generated upon gastric pepsin cleavage of lactoferrin. We have examined the interaction of lactoferricin of bovine origin, Lf-cin B, with the antibiotics penicillin G, vancomycin, gentamicin, colistin, D-cycloserine and erythromycin against E. coli ATCC 25922 and Staphylococcus aureus ATCC 25923. We demonstrated synergism between Lf-cin B and erythromycin against E. coli, and partial synergism between Lf-cin B and penicillin G, vancomycin and gentamicin against E. coli. Only penicillin G acted in partial synergism with Lf-cin B against S. aureus. Lf-cin B antagonized vancomycin and gentamicin against S. aureus in low concentration. We conclude that Lf-cin B may facilitate the uptake of antibiotics across the cell envelope.

摘要

抗菌肽乳铁传递素可通过乳铁蛋白经胃蛋白酶裂解产生。我们研究了源自牛的乳铁传递素Lf-cin B与抗生素青霉素G、万古霉素、庆大霉素、黏菌素、D-环丝氨酸和红霉素针对大肠杆菌ATCC 25922和金黄色葡萄球菌ATCC 25923的相互作用。我们证明了Lf-cin B与红霉素针对大肠杆菌具有协同作用，且Lf-cin B与青霉素G、万古霉素和庆大霉素针对大肠杆菌具有部分协同作用。仅青霉素G与Lf-cin B针对金黄色葡萄球菌具有部分协同作用。低浓度时，Lf-cin B对金黄色葡萄球菌拮抗万古霉素和庆大霉素。我们得出结论，Lf-cin B可能促进抗生素穿过细胞膜。