Kazuno M, Tokunaga T, Oshika Y, Tanaka Y, Tsugane R, Kijima H, Yamazaki H, Ueyama Y, Nakamura M
Department of Neurosurgery, Tokai University, School of Medicine, Kanagawa, Japan.
Eur J Cancer. 1999 Mar;35(3):502-6. doi: 10.1016/s0959-8049(98)00374-8.
Thrombospondins (TSPs) are angiostatic factors in various cancers. However, the significance of TSPs has not been well characterised in glioma. We examined TSP1, TSP2 and vascular endothelial growth factor (VEGF) gene expression by reverse transcription-polymerase chain reaction (RT-PCR) in 37 gliomas. Thirty of the 37 glioma specimens showed VEGF gene expression. Eighteen of the 37 gliomas expressed the TSP1 gene. Seven gliomas lacked TSP2 gene expression, while the other 30 expressed TSP2. The lack of TSP2 gene expression was significantly associated with higher histological grade (Fisher's test, P = 0.0019) and increased vessel counts and density (Student's t-test, P < 0.0001), while there were no correlations between TSP1 and VEGF gene expression and clinicopathological features. These results indicate that the lack of TSP2 gene expression is a potent factor for enhancement of angiogenesis in glioma.
血小板反应蛋白（TSPs）是多种癌症中的血管生成抑制因子。然而，TSPs在胶质瘤中的意义尚未得到充分阐明。我们通过逆转录聚合酶链反应（RT-PCR）检测了37例胶质瘤中TSP1、TSP2和血管内皮生长因子（VEGF）的基因表达。37例胶质瘤标本中有30例显示VEGF基因表达。37例胶质瘤中有18例表达TSP1基因。7例胶质瘤缺乏TSP2基因表达，其余30例表达TSP2。TSP2基因表达的缺失与更高的组织学分级显著相关（Fisher检验，P = 0.0019），并且血管计数和密度增加（Student t检验，P < 0.0001），而TSP1和VEGF基因表达与临床病理特征之间没有相关性。这些结果表明，TSP2基因表达的缺失是胶质瘤血管生成增强的一个重要因素。
