Kim S E, Lee Y H, Park J H, Lee S K
College of Pharmacy, Seoul National University, Korea.
Eur J Cancer. 1999 Mar;35(3):507-11. doi: 10.1016/s0959-8049(98)00415-8.
In this paper, we present evidence that ginsenoside-Rs4 (G-Rs4; an acetylated analogue of ginsenoside-Rg5), a new ginseng saponin isolated from Panax ginseng C. A. Meyer, elevates protein levels of p53 and p21WAF1, which are associated with the induction of apoptosis in SK-HEP-1 cells. Flow cytometric analyses showed that G-Rs4 initially arrested the cell cycle at the G1/S boundary, but consequently induced apoptosis as evidenced by generating an apoptotic peak. The induction of apoptosis was confirmed by the results of DNA fragmentation assays and alterations in cell morphology after treatment of the cells with G-Rs4. Immunoblot assays showed that G-Rs4 significantly elevated protein levels of p53 and p21WAF1, concurrently with the downregulation of both cyclins E- and A-dependent kinase activities and induction of apoptosis. We suggest that G-Rs4 induces apoptosis, the effect of which is closely related to the downregulation of both cyclins E- and A-dependent kinase activity as a consequence of selectively elevating protein levels of p53 and p21WAF1 in SK-HEP-1 cells.
在本文中，我们提供证据表明，从人参（Panax ginseng C. A. Meyer）中分离出的一种新的人参皂苷——人参皂苷-Rs4（G-Rs4；人参皂苷-Rg5的乙酰化类似物），可提高p53和p21WAF1的蛋白水平，这与SK-HEP-1细胞凋亡的诱导有关。流式细胞术分析表明，G-Rs4最初使细胞周期停滞在G1/S边界，但随后诱导了凋亡，这通过产生凋亡峰得以证明。在用G-Rs4处理细胞后，DNA片段化分析结果和细胞形态变化证实了凋亡的诱导。免疫印迹分析表明，G-Rs4显著提高了p53和p21WAF1的蛋白水平，同时下调了细胞周期蛋白E和A依赖性激酶的活性并诱导了凋亡。我们认为，G-Rs4诱导凋亡，其作用与通过选择性提高SK-HEP-1细胞中p53和p21WAF1的蛋白水平从而下调细胞周期蛋白E和A依赖性激酶活性密切相关。
