Traber M G, Arai H
Linus Pauling Institute, Department of Nutrition and Food Management, Oregon State University, Corvallis 97331-6512, USA.
Annu Rev Nutr. 1999;19:343-55. doi: 10.1146/annurev.nutr.19.1.343.
If the function of vitamin E is that of an antioxidant and the various forms of vitamin E have similar antioxidant activities, then why does RRR-alpha-tocopherol have the highest biologic activity? This chapter describes how interactions by investigators from various scientific disciplines using stable isotopes, molecular biology tools, and sophisticated genetic studies of humans with vitamin E deficiency have led to an understanding of this problem. This chapter provides an overview of (a) studies using deuterated tocopherols that demonstrated that the plasma preference for alpha-tocopherol is dependent on metabolic processes in the liver; (b) the isolation, molecular biology, and function of the alpha-tocopherol transfer protein; and (c) studies that demonstrated that patients who were vitamin E deficient as a result of no known cause had defective alpha-tocopherol transfer protein genes. Finally, we focus on the future--what remains to be learned about the regulation of vitamin E in tissues.
如果维生素E的功能是抗氧化剂，并且各种形式的维生素E具有相似的抗氧化活性，那么为什么RRR-α-生育酚具有最高的生物活性呢？本章描述了来自不同科学学科的研究人员如何通过使用稳定同位素、分子生物学工具以及对维生素E缺乏症患者进行复杂的基因研究，从而对这个问题有了深入了解。本章概述了：(a) 使用氘代生育酚的研究，这些研究表明血浆对α-生育酚的偏好取决于肝脏中的代谢过程；(b) α-生育酚转运蛋白的分离、分子生物学和功能；(c) 研究表明，因不明原因导致维生素E缺乏的患者其α-生育酚转运蛋白基因存在缺陷。最后，我们关注未来——关于组织中维生素E的调节仍有待了解的内容。
