Mohn Emily S., Kuchan Matthew J., Erdman John W., Neuringer Martha, Matthan Nirupa R., Chen Chung-Yen Oliver, Johnson Elizabeth J.
Jean Mayer US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA;
Discovery Research, Abbott Nutrition, Columbus, OH 43229, USA;
Antioxidants (Basel). 2017 Nov 26;6(4):97. doi: 10.3390/antiox6040097.
The relationship between α-tocopherol, a known antioxidant, and polyunsaturated fatty acid (PUFA) oxidation, has not been directly investigated in the primate brain. This study characterized the membrane distribution of α-tocopherol in brain regions and investigated the association between membrane α-tocopherol and PUFA content, as well as brain PUFA oxidation products. Nuclear, myelin, mitochondrial, and neuronal membranes were isolated using a density gradient from the prefrontal cortex (PFC), cerebellum (CER), striatum (ST), and hippocampus (HC) of adult rhesus monkeys ( = 9), fed a stock diet containing vitamin E (α-, γ-tocopherol intake: ~0.7 µmol/kg body weight/day, ~5 µmol/kg body weight/day, respectively). α-tocopherol, PUFAs, and PUFA oxidation products were measured using high performance liquid chromatography (HPLC), gas chromatography (GC) and liquid chromatography-gas chromatography/mass spectrometry (LC-GC/MS) respectively. α-Tocopherol (ng/mg protein) was highest in nuclear membranes ( < 0.05) for all regions except HC. In PFC and ST, arachidonic acid (AA, µg/mg protein) had a similar membrane distribution to α-tocopherol. Total α-tocopherol concentrations were inversely associated with AA oxidation products (isoprostanes) ( < 0.05), but not docosahexaenoic acid oxidation products (neuroprostanes). This study reports novel data on α-tocopherol accumulation in primate brain regions and membranes and provides evidence that α-tocopherol and AA are similarly distributed in PFC and ST membranes, which may reflect a protective effect of α-tocopherol against AA oxidation.
已知的抗氧化剂α-生育酚与灵长类动物大脑中多不饱和脂肪酸(PUFA)氧化之间的关系尚未得到直接研究。本研究对大脑区域中α-生育酚的膜分布进行了表征,并研究了膜α-生育酚与PUFA含量以及脑PUFA氧化产物之间的关联。使用密度梯度从成年恒河猴(n = 9)的前额叶皮质(PFC)、小脑(CER)、纹状体(ST)和海马体(HC)中分离出核膜、髓鞘膜、线粒体膜和神经元膜,这些猴子喂食含有维生素E的常规饮食(α-生育酚、γ-生育酚摄入量分别约为0.7 μmol/千克体重/天、5 μmol/千克体重/天)。分别使用高效液相色谱法(HPLC)、气相色谱法(GC)和液相色谱-气相色谱/质谱联用仪(LC-GC/MS)测量α-生育酚、PUFA和PUFA氧化产物。除HC外,所有区域的核膜中α-生育酚(纳克/毫克蛋白质)含量最高(P < 0.05)。在PFC和ST中,花生四烯酸(AA,微克/毫克蛋白质)的膜分布与α-生育酚相似。总α-生育酚浓度与AA氧化产物(异前列腺素)呈负相关(P < 0.05),但与二十二碳六烯酸氧化产物(神经前列腺素)无关。本研究报告了关于α-生育酚在灵长类动物大脑区域和膜中积累的新数据,并提供证据表明α-生育酚和AA在PFC和ST膜中分布相似,这可能反映了α-生育酚对AA氧化的保护作用。