Krendel M, Gloushankova N A, Bonder E M, Feder H H, Vasiliev J M, Gelfand I M
Program in Cellular and Molecular Biodynamics, Rutgers University, Newark, NJ 07102, USA.
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9666-70. doi: 10.1073/pnas.96.17.9666.
The spatial organization of cell-cell adherens junctions is distinct in cultured cells from two different tissue types, specifically, epitheliocytes and fibroblasts. In epitheliocytes, contacts are localized tangentially, along contacting cell edges and in association with circumferential actin bundles. Contacts between fibroblasts are radially oriented; that is, they are perpendicular to the overlapping edges of the cells and are associated with straight bundles of actin filaments. In the present study, we establish that the spatial organization of cell-cell contacts in the epithelial cell line IAR-2 can be converted from the typical tangential pattern to the radial pattern observed in fibroblasts. This transition can be induced by treatment with two agents, phorbol 12-myristate 13-acetate and nocodazole, which have different modes of action. Inhibition of myosin contractility reverses tangential-to-radial conversion of cell-cell contacts. These data suggest that formation of radially aligned contacts depends on modulation of contractility within the actin cytoskeleton through the myosin motor protein. The results open the possibility that modulation of the spatial organization of cell-cell contacts may play important roles in regulating organization and physiological functions of epithelial tissues.
来自两种不同组织类型（即上皮细胞和成纤维细胞）的培养细胞中，细胞间黏附连接的空间组织是不同的。在上皮细胞中，接触沿切线方向定位，沿着接触的细胞边缘并与周向肌动蛋白束相关。成纤维细胞之间的接触呈放射状排列；也就是说，它们垂直于细胞的重叠边缘，并与肌动蛋白丝的直束相关。在本研究中，我们确定上皮细胞系IAR-2中细胞间接触的空间组织可以从典型的切线模式转变为在成纤维细胞中观察到的放射状模式。这种转变可以由两种作用方式不同的试剂（佛波醇12-肉豆蔻酸酯13-乙酸酯和诺考达唑）处理诱导。肌球蛋白收缩性的抑制可逆转细胞间接触从切线状到放射状的转变。这些数据表明，放射状排列接触的形成取决于通过肌球蛋白运动蛋白对肌动蛋白细胞骨架内收缩性的调节。这些结果开启了一种可能性，即细胞间接触空间组织的调节可能在调节上皮组织的组织和生理功能中发挥重要作用。
