Tanei R, Ohta Y, Ishihara S, Katsuoka K, Yokono H, Motoori T
Department of Dermatology, School of Medicine, Kitasato University, Kanagawa, Japan.
Dermatology. 1999;199(1):20-4. doi: 10.1159/000018172.
Acute graft-versus-host disease (GVHD) can occur after a blood transfusion.
In order to elucidate the pathomechanisms responsible for transfusion-associated GVHD, infiltrating donor-derived cells in a cutaneous lesion were analyzed.
A skin sample obtained from a 69-year-old woman who developed fatal GVHD after blood transfusions from male donors was studied by performing in situ hybridization (ISH) with a Y-chromosome-specific probe.
The cell infiltrates comprised mainly CD3+ T lymphocytes. Immunohistochemistry and ISH in combination demonstrated that 99% (182/184) of the Y-body-positive cells were CD3+. Y bodies were observed in 80% of the CD8+ cells in the epidermis and dermoepidermal junction and in 77 and 45% of the CD8+ and CD4+ cells, respectively, in the dermis.
These findings suggest that both CD4+ and CD8+ cells of donor origin were involved in the development of cutaneous GVHD.
急性移植物抗宿主病（GVHD）可在输血后发生。
为阐明输血相关GVHD的发病机制，对皮肤病变中浸润的供体来源细胞进行分析。
对一名69岁女性的皮肤样本进行研究，该女性在接受男性供体输血后发生致命性GVHD，采用Y染色体特异性探针进行原位杂交（ISH）。
细胞浸润主要由CD3 + T淋巴细胞组成。免疫组织化学和ISH联合显示，99%（182/184）的Y体阳性细胞为CD3 +。在表皮和真皮表皮交界处，80%的CD8 +细胞中观察到Y体，在真皮中，CD8 +和CD4 +细胞中分别有77%和45%观察到Y体。
这些发现表明，供体来源的CD4 +和CD8 +细胞均参与了皮肤GVHD的发生。
