Mráz J, Gálová E, Nohová H, Vítková D, Tichý M
National Institute of Public Health, Centre of Industrial Hygiene and Occupational Diseases, Prague, Czech Republic.
Scand J Work Environ Health. 1999 Jun;25(3):233-7. doi: 10.5271/sjweh.429.
This study explored the acute effect of ethanol (EtOH) on the urinary excretion of cyclohexanol (CH-ol), 1,2- and 1,4-cyclohexanediol (CH-diol), biomarkers of exposure to important solvents, and chemical intermediates cyclohexanone (CH-one), cyclohexane (CH) and cyclohexanol.
Volunteers (5-8 in each group) were exposed for 8 hours either to CH-one, CH or CH-ol vapor at concentrations of about 200, 1000, and 200 mg/m3, respectively, with concomitant ingestion of EtOH (4 14-g doses taken during the exposure). Urine was collected for 72 hours and analyzed for CH-ol and CH-diols using a procedure involving acidic hydrolysis and gas chromatographic determination.
The metabolic yields of CH-ol, 1,2-, and 1,4-CH-diol, respectively, in the exposures with EtOH were as follows: 11.3%, 36%, 23% after the exposure to CH-one, 3.1%, 15%, 8% after the exposure to CH, and 6.6%, 24%, 18% after the exposure to CH-ol. [The corresponding values obtained previously in matching experiments without EtOH were as follows: 1.0%, 39%, 18% (CH-one); 0.5%, 23%, 11% (CH); and 1.1%, 19%, 8% (CH-ol).] The excretion curves of the metabolites in the exposures with EtOH were not delayed when compared with the corresponding curves of a comparison group.
The urinary excretion of CH-diols is much less sensitive to EtOH than that of CH-ol. It is recommended to employ CH-diols as useful and more reliable biomarkers of exposure to CH-one, CH and CH-ol in field examinations.
本研究探讨了乙醇（EtOH）对环己醇（CH - ol）、1,2 - 和1,4 - 环己二醇（CH - diol）（重要溶剂暴露的生物标志物）以及化学中间体环己酮（CH - one）、环己烷（CH）和环己醇的尿排泄的急性影响。
志愿者（每组5 - 8人）分别暴露于浓度约为200、1000和200 mg/m³的CH - one、CH或CH - ol蒸气中8小时，同时摄入EtOH（暴露期间分4次服用14 - g剂量）。收集尿液72小时，并采用酸性水解和气相色谱测定法分析CH - ol和CH - diols。
在乙醇暴露实验中，CH - ol、1,2 - 和1,4 - CH - diol的代谢产率分别如下：暴露于CH - one后为11.3%、36%、23%；暴露于CH后为3.1%、15%、8%；暴露于CH - ol后为6.6%、24%、18%。[之前在无乙醇的匹配实验中获得的相应值如下：（CH - one）1.0%、39%、18%；（CH）0.5%、23%、11%；（CH - ol）1.1%、19%、8%。]与对照组的相应曲线相比，乙醇暴露实验中代谢物的排泄曲线没有延迟。
CH - diols的尿排泄对EtOH的敏感性远低于CH - ol。建议在现场检查中采用CH - diols作为暴露于CH - one、CH和CH - ol的有用且更可靠的生物标志物。
