Mráz J, Gálová E, Nohová H, Vítková D
National Institute of Public Health, Prague, Czech Republic.
Int Arch Occup Environ Health. 1994;66(3):203-8. doi: 10.1007/BF00380781.
The metabolism and toxicokinetics of cyclohexanone (CH-one), an important solvent and chemical intermediate, have been studied in volunteers during and after 8-h exposures to CH-one vapour at a concentration of 101, 207 and 406 mg.m-3. The pulmonary ventilation in these experiments was typically 11 l.min-1 and retention in the respiratory tract was 58%. After exposure to CH-one, 207 mg.m-3, the metabolic yields of cyclohexanol (CH-ol), 1,2- and 1,4-cyclohexanediol (CH-diol) as determined in urine by a gas chromatographic method involving hydrolysis of glucuronide conjugate were 1.0% +/- 0.3%, 39% +/- 5% and 18% +/- 2% (n = 8), respectively. Peak excretion of CH-ol was achieved at the end of the exposure period, after which it decayed rapidly. Elimination of 1,2- and 1,4-CH-diol reached maximum values a few hours following exposure, with subsequent elimination half-times of 16 +/- 2 and 18 +/- 4 h, respectively. Repeated exposure to CH-one vapour (around 200 mg.m-3) for five consecutive days (8 h/day) resulted in cumulative excretion of CH-diols. The permeation rate of CH-one liquid through the skin was 0.037-0.069 mg.cm-2.h-1 (n = 3), indicating that the contribution of percutaneous absorption to total CH-one occupational intake is of minor importance. CH-diols are recommended as biomarkers of exposure to CH-one.
环己酮（CH - one）是一种重要的溶剂和化学中间体，研究人员让志愿者在8小时内暴露于浓度分别为101、207和406 mg·m⁻³的CH - one蒸气中，并在暴露期间及之后对其代谢和毒代动力学进行了研究。这些实验中的肺通气量通常为11 l·min⁻¹，呼吸道滞留率为58%。暴露于207 mg·m⁻³的CH - one后，通过涉及葡萄糖醛酸共轭物水解的气相色谱法测定，尿液中环己醇（CH - ol）、1,2 - 和1,4 - 环己二醇（CH - diol）的代谢产率分别为1.0%±0.3%、39%±5%和18%±2%（n = 8）。CH - ol在暴露期结束时达到排泄峰值，之后迅速下降。1,2 - 和1,4 - CH - diol的排泄在暴露后数小时达到最大值，随后的消除半衰期分别为16±2小时和18±4小时。连续五天（每天8小时）重复暴露于CH - one蒸气（约200 mg·m⁻³）导致CH - diols的累积排泄。CH - one液体通过皮肤的渗透速率为0.037 - 0.069 mg·cm⁻²·h⁻¹（n = 3），表明经皮吸收对CH - one职业摄入量的贡献较小。建议将CH - diols作为暴露于CH - one的生物标志物。
