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1
Exonuclease associated with bacteriophage T5-Induced DNA polymerase.与噬菌体T5诱导的DNA聚合酶相关的核酸外切酶。
J Virol. 1976 Oct;20(1):70-7. doi: 10.1128/JVI.20.1.70-77.1976.
2
Exonuclease-polymerase active site partitioning of primer-template DNA strands and equilibrium Mg2+ binding properties of bacteriophage T4 DNA polymerase.噬菌体T4 DNA聚合酶的引物-模板DNA链的核酸外切酶-聚合酶活性位点分区及Mg2+平衡结合特性
Biochemistry. 1998 Jul 14;37(28):10144-55. doi: 10.1021/bi980074b.
3
Temperature-sensitive DNA polymerase induced by a bacteriophage T5 mutant: relationship between polymerase and exonuclease activities.噬菌体T5突变体诱导的温度敏感型DNA聚合酶：聚合酶与核酸外切酶活性之间的关系。
Biochemistry. 1976 Oct 5;15(20):4403-9. doi: 10.1021/bi00665a009.
4
Primer-mediated inhibition of the hydrolysis of template DNA by T5-induced DNA polymerase.
Biochem Biophys Res Commun. 1977 Nov 7;79(1):247-53. doi: 10.1016/0006-291x(77)90087-0.
5
In vivo effects of recBC DNase, exonuclease I, and DNA polymerases of Escherichia coli on the infectivity of native and single-stranded DNA of bacteriophage T7.大肠杆菌的recBC核酸酶、核酸外切酶I和DNA聚合酶对噬菌体T7天然DNA和单链DNA感染性的体内效应
J Virol. 1977 Mar;21(3):906-12. doi: 10.1128/JVI.21.3.906-912.1977.
6
Mechanism of 3' to 5' exonuclease associated with phage T5-induced DNA polymerase: processiveness and template specificity.与噬菌体T5诱导的DNA聚合酶相关的3'至5'核酸外切酶的机制：持续合成能力和模板特异性。
Nucleic Acids Res. 1980 Feb 11;8(3):657-71. doi: 10.1093/nar/8.3.657.
7
An exonuclease activity associated with DNA polymerase I of Micrococcus radiodurans.与耐辐射微球菌DNA聚合酶I相关的核酸外切酶活性。
Biochim Biophys Acta. 1978 Aug 23;520(1):122-30. doi: 10.1016/0005-2787(78)90013-8.
8
Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase.T5 DNA聚合酶介导的引物-模板依赖性dNTP转化为dNMP的机制。
J Biol Chem. 1980 Aug 10;255(15):7149-54.
9
Characterization of the 5' to 3' exonuclease associated with Thermus aquaticus DNA polymerase.与嗜热栖热菌DNA聚合酶相关的5'至3'核酸外切酶的特性分析。
Nucleic Acids Res. 1990 Dec 25;18(24):7317-22. doi: 10.1093/nar/18.24.7317.
10
5' leads to 3'-Exonucleases of bacteriophage T4.
J Biol Chem. 1976 May 10;251(9):2613-9.

引用本文的文献

1
Interaction of a DNA-binding protein, the product of gene D5 of bacteriophage T5, with double-stranded DNA: effects on T5 DNA polymerase functions in vitro.噬菌体T5基因D5产物（一种DNA结合蛋白）与双链DNA的相互作用：对T5 DNA聚合酶体外功能的影响
J Virol. 1983 Jun;46(3):778-87. doi: 10.1128/JVI.46.3.778-787.1983.
2
DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.原核生物和真核生物中的DNA聚合酶：作用模式及生物学意义
Experientia. 1983 Jan 15;39(1):1-25. doi: 10.1007/BF01960616.
3
Restriction map of the single-stranded DNA genome of Kilham rat virus strains 171, a nondefective parvovirus.基尔汉姆大鼠病毒171株单链DNA基因组的限制酶切图谱，171株是一种无缺陷细小病毒。
J Virol. 1981 Oct;40(1):118-25. doi: 10.1128/JVI.40.1.118-125.1981.
4
Mechanism of 3' to 5' exonuclease associated with phage T5-induced DNA polymerase: processiveness and template specificity.与噬菌体T5诱导的DNA聚合酶相关的3'至5'核酸外切酶的机制：持续合成能力和模板特异性。
Nucleic Acids Res. 1980 Feb 11;8(3):657-71. doi: 10.1093/nar/8.3.657.

本文引用的文献

1
Separation of the phosphoric esters on the filter paper chromatogram.磷酸酯在滤纸色谱上的分离
Nature. 1949 Dec 31;164(4183):1107-12, illust. doi: 10.1038/1641107a0.
2
The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease.大肠杆菌的脱氧核糖核酸酶。II. 一种核糖核酸抑制性内切核酸酶的纯化及特性
J Biol Chem. 1962 Mar;237:819-28.
3
Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4.用噬菌体T4的一些琥珀突变体感染大肠杆菌后早期酶的合成及其调控
Proc Natl Acad Sci U S A. 1962 Feb;48(2):293-302. doi: 10.1073/pnas.48.2.293.
4
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.脱氧核糖核酸的酶促合成。IX. T2噬菌体感染大肠杆菌后形成的聚合酶：一种新酶。
J Biol Chem. 1962 Feb;237:519-25.
5
The enzymology of virus-infected bacteria. VII. A new deoxyribonucleic acid polymerase induced by bacteriophage T5.病毒感染细菌的酶学。VII. 噬菌体T5诱导的一种新的脱氧核糖核酸聚合酶。
J Biol Chem. 1965 Dec;240(12):4652-8.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
7
Enzymatic breakage and joining of deoxyribonucleic acid. V. End group labeling and analysis of deoxyribonucleic acid containing single straned breaks.脱氧核糖核酸的酶促断裂与连接。V. 末端基团标记及含单链断裂的脱氧核糖核酸分析
J Biol Chem. 1968 Sep 10;243(17):4530-42.
8
Nucleotide analysis of deoxyribonucleic acid containing deoxybromouridylic acid.含脱氧溴尿苷酸的脱氧核糖核酸的核苷酸分析。
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9
Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.来自T4噬菌体感染的大肠杆菌的一种酶对核酸的磷酸化作用。
Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65. doi: 10.1073/pnas.54.1.158.
10
The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5.噬菌体T4和T5中脱氧核糖核酸聚合酶的结构基因。
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241-8. doi: 10.1073/pnas.54.4.1241.

与噬菌体T5诱导的DNA聚合酶相关的核酸外切酶。

Exonuclease associated with bacteriophage T5-Induced DNA polymerase.

作者信息

Das S K, Fujimura R K

出版信息

J Virol. 1976 Oct;20(1):70-7. doi: 10.1128/JVI.20.1.70-77.1976.

DOI:10.1128/JVI.20.1.70-77.1976
PMID:10451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354967/
Abstract

T-5-induced DNA polymerase has been shown to possess a 3' leads to 5'-exonucleolytic activity. The exonuclease acts on both native and denatured DNA, but the apparent rate of degradation of denatured DNA is about five times faster than that for native DNA. The enzyme appears to act only on 3'-OH ends and produces mainly 5'-dNMP's. Like polymerase activity, exonuclease activity shows a pH optimum around 8.6. Mg2+, dithiothreitol, and N-ethylmaleimide had identical effects on both the activities. Nicked DNA was almost totally protected from exonuclease action under synthetic conditions, i.e., in the presence of 4dNTP's. Denatured DNA was partly degraded in the early phase of incubation with 4dNTP's, presumably due to unhybridized tails at the 3'-OH primer ends. However, the exonuclease activity was operative in both cases under synthetic conditions, as evidenced by template-dependent conversion of [3H]dTTP to [3H]dTMP.

摘要

已证明T-5诱导的DNA聚合酶具有3'至5'核酸外切酶活性。该核酸外切酶作用于天然DNA和变性DNA，但变性DNA的表观降解速率比天然DNA快约五倍。该酶似乎仅作用于3'-OH末端，主要产生5'-dNMP。与聚合酶活性一样，核酸外切酶活性在pH约8.6时显示最佳值。Mg2+、二硫苏糖醇和N-乙基马来酰亚胺对这两种活性具有相同的影响。在合成条件下，即存在4种dNTP时，带切口的DNA几乎完全免受核酸外切酶作用。在与4种dNTP孵育的早期阶段，变性DNA部分降解，可能是由于3'-OH引物末端未杂交的尾巴。然而，在合成条件下，两种情况下核酸外切酶活性均起作用，这由[3H]dTTP向[3H]dTMP的模板依赖性转化证明。