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1
Interaction of a DNA-binding protein, the product of gene D5 of bacteriophage T5, with double-stranded DNA: effects on T5 DNA polymerase functions in vitro.噬菌体T5基因D5产物（一种DNA结合蛋白）与双链DNA的相互作用：对T5 DNA聚合酶体外功能的影响
J Virol. 1983 Jun;46(3):778-87. doi: 10.1128/JVI.46.3.778-787.1983.
2
Interaction of a DNA-binding protein, the gene product of D5 of bacteriophage T5, with double-stranded DNA. Analysis by metrizamide gradient centrifugation.
J Biol Chem. 1982 Dec 25;257(24):14811-6.
3
Isolation and characterization of a putative bacteriophage T5 transcription.replication enzyme complex from infected Escherichia coli.从感染的大肠杆菌中分离和鉴定一种假定的噬菌体T5转录-复制酶复合物
J Biol Chem. 1980 Jul 25;255(14):7040-8.
4
The purification and properties of a double-stranded DNA-binding protein encoded by the gene D5 of bacteriophage T5.噬菌体T5基因D5编码的双链DNA结合蛋白的纯化及特性
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On the role of the single-stranded DNA binding protein of bacteriophage T4 in DNA metabolism. I. Isolation and genetic characterization of new mutations in gene 32 of bacteriophage T4.噬菌体T4单链DNA结合蛋白在DNA代谢中的作用。I. 噬菌体T4基因32新突变的分离与遗传特征分析
Mol Gen Genet. 1982;188(1):77-90. doi: 10.1007/BF00332998.
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Mechanism of 3' to 5' exonuclease associated with phage T5-induced DNA polymerase: processiveness and template specificity.与噬菌体T5诱导的DNA聚合酶相关的3'至5'核酸外切酶的机制：持续合成能力和模板特异性。
Nucleic Acids Res. 1980 Feb 11;8(3):657-71. doi: 10.1093/nar/8.3.657.
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A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease.一个允许单链DNA穿过T5 5'-外切核酸酶的螺旋状拱。
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10
Exonuclease-polymerase active site partitioning of primer-template DNA strands and equilibrium Mg2+ binding properties of bacteriophage T4 DNA polymerase.噬菌体T4 DNA聚合酶的引物-模板DNA链的核酸外切酶-聚合酶活性位点分区及Mg2+平衡结合特性
Biochemistry. 1998 Jul 14;37(28):10144-55. doi: 10.1021/bi980074b.

引用本文的文献

1
Identification by immunobinding assay of the polypeptide coded by the DNA polymerase gene of bacteriophage T5 and its amber mutants and the direction of transcription of the gene.通过免疫结合测定法鉴定噬菌体T5的DNA聚合酶基因及其琥珀突变体所编码的多肽以及该基因的转录方向。
J Virol. 1985 Oct;56(1):245-9. doi: 10.1128/JVI.56.1.245-249.1985.

本文引用的文献

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How the lambda repressor and cro work.λ阻遏蛋白和Cro蛋白是如何发挥作用的。
Cell. 1980 Jan;19(1):1-11. doi: 10.1016/0092-8674(80)90383-9.
2
Interaction of a DNA-binding protein, the gene product of D5 of bacteriophage T5, with double-stranded DNA. Analysis by metrizamide gradient centrifugation.
J Biol Chem. 1982 Dec 25;257(24):14811-6.
3
A small subclass of SV40 T antigen binds to the viral origin of replication.SV40 T抗原的一个小亚类与病毒复制起点结合。
Cell. 1982 Jun;29(2):375-83. doi: 10.1016/0092-8674(82)90154-4.
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Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin.
Nucleic Acids Res. 1981 Apr 24;9(8):1789-99. doi: 10.1093/nar/9.8.1789.
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Replication origins in the eucaryotic chromosome.真核染色体中的复制起点。
Cell. 1981 May;24(2):283-4. doi: 10.1016/0092-8674(81)90316-0.
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Replicative DNA polymerases and mechanisms at a replication fork.
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7
Mechanism of primer-template-dependent conversion of dNTP leads to dNMP by T5 DNA polymerase.T5 DNA聚合酶介导的引物-模板依赖性dNTP转化为dNMP的机制。
J Biol Chem. 1980 Aug 10;255(15):7149-54.
8
Isolation and characterization of a putative bacteriophage T5 transcription.replication enzyme complex from infected Escherichia coli.从感染的大肠杆菌中分离和鉴定一种假定的噬菌体T5转录-复制酶复合物
J Biol Chem. 1980 Jul 25;255(14):7040-8.
9
The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.噬菌体T5的染色体。I. 用琼脂糖凝胶电泳分析单链DNA片段
J Mol Biol. 1972 Feb 14;63(3):383-95. doi: 10.1016/0022-2836(72)90435-4.
10
Biochemical analysis of the naturally repaired sections of bacteriophage T5 deoxyribonucleic acid. I. Bromodeoxyuridine incorporation into parental deoxyribonucleic acid in the absence of deoxyribonucleic acid replication.
Biochemistry. 1968 Oct;7(10):3488-98. doi: 10.1021/bi00850a025.

噬菌体T5基因D5产物（一种DNA结合蛋白）与双链DNA的相互作用：对T5 DNA聚合酶体外功能的影响

Interaction of a DNA-binding protein, the product of gene D5 of bacteriophage T5, with double-stranded DNA: effects on T5 DNA polymerase functions in vitro.

作者信息

Fujimura R K, Roop B C

出版信息

J Virol. 1983 Jun;46(3):778-87. doi: 10.1128/JVI.46.3.778-787.1983.

DOI:10.1128/JVI.46.3.778-787.1983
PMID:6304341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256554/
Abstract

The gene D5 product (gpD5) of bacteriophage T5 is a DNA-binding protein that binds preferentially to double-stranded DNA and is essential for T5 DNA replication, yet it inhibits DNA synthesis in vitro. Mechanisms of inhibition were studied by using nicked DNA and primed single-stranded DNA as a primer-template. Inhibition of T5 DNA polymerase activity by gpD5 occurred when double-stranded regions of DNA were saturated with gpD5. The 3' leads to 5' exonuclease associated with T5 DNA polymerase was not very active with nicked DNA, but inhibition of hydrolysis of substituents at 3'-hydroxyl termini by gpD5 could be observed. T5 DNA polymerase appears to be capable of binding to the 3' termini even when double-stranded regions are saturated with gpD5. The interaction of gpD5 with the polymerases at the primer terminus is apparently the primary cause of inhibition of polymerization.

摘要

噬菌体T5的基因D5产物（gpD5）是一种DNA结合蛋白，它优先结合双链DNA，对T5 DNA复制至关重要，但在体外却抑制DNA合成。利用带切口的DNA和带引物的单链DNA作为引物模板研究了抑制机制。当DNA的双链区域被gpD5饱和时，gpD5会抑制T5 DNA聚合酶的活性。与T5 DNA聚合酶相关的3'→5'核酸外切酶对带切口的DNA活性不是很强，但可以观察到gpD5对3'-羟基末端取代基水解的抑制作用。即使双链区域被gpD5饱和，T5 DNA聚合酶似乎仍能够结合到3'末端。gpD5与引物末端的聚合酶之间的相互作用显然是聚合抑制的主要原因。