Fang P, Ding R, Kong L
Shanghai Burns Institute, Rui Jin Hospital Affiliated to Shanghai Second Medical University.
Zhonghua Zheng Xing Shao Shang Wai Ke Za Zhi. 1997 Jul;13(4):259-63.
We observed the changes in wound histopathology and fibrinolysis of rats with deep partial thickness burns during 10 days after scalding in order to study the relations of early progressive damage of deep partial thickness burn wound and the healing of the wound to fibrinolysis. Using Masson's trichrome stain of collagen, we demonstrated that partial thickness burn wound progressively deteriorated within 72 hours postburn, and epidermal cells on wound edge proliferated, creeping between necrotic and residual collagen by 10th day postburn. The results of assays of fibrinolytic parameters in plasma and 24 hour exudate from wound showed that fibrinolysis activated at 2 hour postburn but had been suppressed by 24 hour postburn till last day (10 day postburn) of the observation. The analysis of dynamic changes and relations in fibrinolytic parameters revealed that the suppressive factors of fibrinolysis might be enhanced activities of PAI, alpha 2-antiplasmin and antithrombin II after injury. Results suggested that suppression of fibrinolysis might protect fibrin deposited in the wound from lysis and play an important role in early progressive deterioration of deep partial thickness burn and regulation of its healing.
为了研究深Ⅱ度烧伤创面早期进行性损害及创面愈合与纤溶的关系，我们观察了深Ⅱ度烫伤大鼠烫伤后10天内创面组织病理学变化及纤溶情况。采用胶原纤维的马松三色染色法，我们发现深Ⅱ度烧伤创面在伤后72小时内逐渐恶化，至伤后第10天，创面边缘的表皮细胞增殖，并在坏死和残留的胶原纤维间爬行。血浆及创面24小时渗出液中纤溶参数的检测结果显示，纤溶在伤后2小时被激活，但在伤后24小时至观察期最后一天（伤后10天）受到抑制。纤溶参数动态变化及相关性分析显示，损伤后纤溶抑制因子可能是纤溶酶原激活物抑制物、α2 -抗纤溶酶及抗凝血酶Ⅲ活性增强。结果提示，纤溶抑制可能保护创面沉积的纤维蛋白不被溶解，在深Ⅱ度烧伤早期进行性恶化及愈合调控中起重要作用。
