鹿茸多肽促进软骨细胞和成骨细胞前体的增殖以及骨折愈合。

Velvet antler polypeptides promoted proliferation of chondrocytes and osteoblast precursors and fracture healing.

作者信息

Zhou Q L, Guo Y J, Wang L J, Wang Y, Liu Y Q, Wang Y, Wang B X

机构信息

Research Centre of New Drug, Changchun College of Traditional Chinese Medicine, China.

出版信息

Zhongguo Yao Li Xue Bao. 1999 Mar;20(3):279-82.

PMID:10452108

Abstract

AIM

To study the effects of velvet antler (VA) total polypeptides (VATP) and VA polypeptides, VAP-A, VAP-B, and VAP-C on proliferation of chondrocytes and osteoblast precusors.

METHODS

Chondrocytes (rabbit and human fetus) and osteoblast precusors (chick embryo) were incubated in the culture medium containing VATP or VAP-A, VAP-B, and VAP-C. [3H]TdR incorporation into DNA was measured. Fracture healing-promoting action of VATP was determined in rats.

RESULTS

VATP 50-200 mg.L-1 and VAP-B 12.5, 25, and 50 mg.L-1 showed most marked proliferation-promoting activity for rabbit costed chondrocytes and increased incorporation of [3H]TdR from (73 +/- 9) Bq (control group) to (272 +/- 55), (327 +/- 38), and (415 +/- 32) Bq, respectively (P < 0.01). The activity of VAP-A was weaker than that of VAP-B, and VAP-C had no activity. VATP 10 and 20 mg.kg-1 by local injection into the cross-section fracture area accelerated healing of radial fracture. The healing rate of VATP-treated group was higher (75%) than that of control group (25%) (P < 0.05).

CONCLUSION

VATP accelerated fracture healing by stimulating proliferation of chondrocytes and osteoblast precursors.

摘要

目的

研究鹿茸（VA）总多肽（VATP）以及VA多肽VAP - A、VAP - B和VAP - C对软骨细胞和成骨细胞前体细胞增殖的影响。

方法

将软骨细胞（兔和人胎儿来源）和成骨细胞前体细胞（鸡胚来源）置于含有VATP或VAP - A、VAP - B和VAP - C的培养基中培养。检测[³H]TdR掺入DNA的情况。在大鼠身上测定VATP促进骨折愈合的作用。

结果

VATP浓度为50 - 200mg·L⁻¹以及VAP - B浓度为12.5、25和50mg·L⁻¹时，对兔肋软骨细胞显示出最显著的促增殖活性，[³H]TdR掺入量分别从（73±9）Bq（对照组）增加到（272±55）、（327±38）和（415±32）Bq（P<0.01）。VAP - A的活性弱于VAP - B，VAP - C无活性。通过局部注射10和20mg·kg⁻¹的VATP到桡骨骨折断面区域，可加速骨折愈合。VATP治疗组的愈合率（75%）高于对照组（25%）（P<0.05）。