同种异体间皮细胞腹腔内移植预防粘连
Intraperitoneal transplantation of isologous mesothelial cells for prevention of adhesions.
作者信息
Bertram P, Tietze L, Hoopmann M, Treutner K H, Mittermayer C, Schumpelick V
机构信息
Department of Surgery, Medical Faculty, Rhenish-Westphalian Technical University, Aachen, Germany.
出版信息
Eur J Surg. 1999 Jul;165(7):705-9. doi: 10.1080/11024159950189780.
OBJECTIVE
To study the effect of transplantation of mesothelial cells on the formation of adhesions after peritoneal abrasion.
DESIGN
Animal study.
SETTING
Teaching hospital, Germany.
ANIMALS
30 isologous eight-week old Lewis rats to allow for harvesting of the greater omentum from a separate group.
INTERVENTIONS
The first group (n = 10) served as mesothelial cell donors. The other animals had laparotomy and induction of adhesions by standardised abrasion of the peritoneum. The trial group (n = 10) were given a suspension of 10(6) mesothelial cells/100 g body weight intraperitoneally and the control group (n = 10) an equal volume of culture medium. After 10 days the animals were killed.
MAIN OUTCOME MEASURES
Measurements of the areas of adhesions by computer aided morphometry.
RESULTS
The trial group developed a mean (SD) adhesion area of 122.7 (176.7) mm2, and the controls 310.5 (179.1) mm2. The corresponding medians (range) were 51.2 (0-547.1) and 274.3 (100.6-575.4). Transplantation of mesothelial cells resulted in a significant reduction in adhesion formation (Wilcoxon test, p<0.01).
CONCLUSION
Intraperitoneal transplantation of mesothelial cells is an effective way of reducing the formation of adhesions.
目的
研究间皮细胞移植对腹膜擦伤后粘连形成的影响。
设计
动物研究。
地点
德国教学医院。
动物
30只同基因8周龄Lewis大鼠，以便从另一组获取大网膜。
干预措施
第一组（n = 10）作为间皮细胞供体。其他动物接受剖腹手术，并通过标准化的腹膜擦伤诱导粘连形成。试验组（n = 10）腹腔内注射10(6)个间皮细胞/100 g体重的悬液，对照组（n = 10）注射等体积的培养基。10天后处死动物。
主要观察指标
通过计算机辅助形态测量法测量粘连面积。
结果
试验组粘连面积的均值（标准差）为122.7（176.7）mm2，对照组为310.5（179.1）mm2。相应的中位数（范围）分别为51.2（0 - 547.1）和274.3（100.6 - 575.4）。间皮细胞移植导致粘连形成显著减少（Wilcoxon检验，p<0.01）。
结论
腹腔内移植间皮细胞是减少粘连形成的有效方法。
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