Su X, Chew F T, Li S F
Institute of Material Research and Engineering, National University of Singapore, Block S7 Level 3, 10 Kent Ridge Crescent, 119260, Singapore.
Anal Biochem. 1999 Aug 15;273(1):66-72. doi: 10.1006/abio.1999.4186.
This work presents a piezoelectric (Pz) immunosensor for the quantification of total human IgE in serum samples. The anti-human IgE is deposited on the surface of the 10 M Hz AT-cut gold coated crystal resonator by self-assembled technique, and serves as a receptor layer. The highly ordered self-assembled monolayers (SAMs) ensure well-controlled surface structure and offer many advantages to the performance of the sensor. The fabricated Pz sensor can quantitatively detect human serum IgE in the range of 5-300 IU/ml with high precision (CV < 8%). A total of 28 patient serum samples are detected by the Pz sensor, and the results agree well with those given by two commercially provided test kits (Total IgE FAST Test, Pharmacia-CAP). The correlation coefficients are 0.94 between FAST and Pz sensor, and 0.90 between CAP and Pz sensor, respectively. After regeneration with urea and glycine buffer the coated crystal can be reused five times without appreciable loss of activity. Compared with conventional cross-linking methods, nonspecific binding caused by the SAM binding method is three to five times less.
这项工作展示了一种用于定量血清样本中总人免疫球蛋白E（IgE）的压电（Pz）免疫传感器。抗人IgE通过自组装技术沉积在10MHz AT切割的镀金晶体谐振器表面，并作为受体层。高度有序的自组装单分子层（SAMs）确保了良好控制的表面结构，并为传感器的性能带来了许多优势。所制备的Pz传感器能够在5 - 300 IU/ml范围内高精度地定量检测人血清IgE（变异系数<8%）。Pz传感器共检测了28份患者血清样本，结果与两种商业提供的检测试剂盒（总IgE快速检测，Pharmacia - CAP）给出的结果高度吻合。FAST与Pz传感器之间的相关系数为0.94，CAP与Pz传感器之间的相关系数分别为0.90。用尿素和甘氨酸缓冲液再生后，涂覆的晶体可重复使用五次，且活性无明显损失。与传统交联方法相比，SAM结合方法引起的非特异性结合减少了三到五倍。
