Yao Chunyan, Chen Qinghai, Chen Ming, Zhang Bo, Luo Yang, Huang Qing, Huang Junfu, Fu Weiling
Department of Clinical Laboratory, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
J Nanosci Nanotechnol. 2006 Dec;6(12):3828-34. doi: 10.1166/jnn.2006.605.
A novel multi-channel 2 x 5 model of piezoelectric (PZ) micro-array immunosensor has been developed for quantitative detection of human immunoglobulinE (IgE) in serum. Every crystal unit of the fabricated piezoelectric IgE micro-array immunosensor can oscillate without interfering each other. A multi-channel 2 x 5 model micro-array immunosensor as compared with the traditional one-channel immunosensor can provide eight times higher detection speeds for IgE assay. The anti-IgE antibody is deposited on the gold electrode's surface of 10 MHz AT-cut quartz crystals by SPA (staphylococcal protein A), and serves as an antibody recognizing layer. The highly ordered antibody monolayers ensure well-controlled surface structure and offer many advantages to the performance of the sensor. The uniform amount of antibody monolayer coated by the SPA is good, and non-specific reaction caused by other immunoglobulin in sample is found. The fabricated PZ immunosensor can be used for human IgE determination in the range of 5-300 IU/ml with high precision (CV is 4%). 50 human serum samples were detected by the micro-array immunosensor, and the results agreed well with those given by the commercially ELISA test kits. The correlation coefficient is 0.94 between ELISA and PZ immunosensor. After regeneration with NaOH the coated immunosensor can be reused 6 times without appreciable loss of activity.
一种新型的多通道2×5模式压电（PZ）微阵列免疫传感器已被开发出来，用于定量检测血清中的人免疫球蛋白E（IgE）。所制备的压电IgE微阵列免疫传感器的每个晶体单元都能独立振荡而互不干扰。与传统的单通道免疫传感器相比，多通道2×5模式微阵列免疫传感器对IgE检测的速度提高了八倍。抗IgE抗体通过葡萄球菌蛋白A（SPA）沉积在10MHz AT切石英晶体的金电极表面，作为抗体识别层。高度有序的抗体单层确保了良好控制的表面结构，并为传感器性能带来诸多优势。通过SPA包被的抗体单层数量均匀，且未发现样品中其他免疫球蛋白引起的非特异性反应。所制备的PZ免疫传感器可用于5-300IU/ml范围内的人IgE测定，精度高（变异系数为4%）。用该微阵列免疫传感器检测了50份人血清样本，结果与市售ELISA检测试剂盒的结果吻合良好。ELISA法与PZ免疫传感器之间的相关系数为0.94。用NaOH再生后，包被的免疫传感器可重复使用6次，活性无明显损失。
