Mason R P, Constantinescu A, Hunjan S, Le D, Hahn E W, Antich P P, Blum C, Peschke P
Advanced Radiological Sciences, University of Texas, Southwestern Medical Center, Dallas 75235-9058, USA.
Radiat Res. 1999 Sep;152(3):239-49.
We recently described a novel approach to measuring regional tumor oxygen tension using (19)F pulse burst saturation recovery (PBSR) nuclear magnetic resonance (NMR) echo planar imaging (EPI) relaxometry of hexafluorobenzene. We now compare oxygen tension measurements in a group of size-matched R3327-AT1 Dunning prostate rat tumors made using this new method with those using a traditional polarographic method: the Eppendorf histograph. Similar oxygen tension distributions were found using the two methods, and both techniques showed that tumors with volume greater than 3.5 cm(3) were significantly (P < 0.0001) less well oxygenated than smaller tumors (volume less than 2 cm(3)). Using the (19)F EPI approach, we also examined the response to respiratory challenge. Increasing the concentration of inspired oxygen from 33% to 100% O(2) produced a significant increase (P < 0.0001) in tumor oxygenation for a group of small tumors. In contrast, no change was observed in the mean pO(2) for a group of large tumors. Consideration of individual tumor regions irrespective of tumor size showed a strong correlation between the maximum pO(2) observed when breathing 100% O(2) compared with mean baseline pO(2). These results further demonstrate the usefulness of (19)F EPI to assess changes in regional tumor oxygenation.
我们最近描述了一种使用六氟苯的（19）F脉冲猝发饱和恢复（PBSR）核磁共振（NMR）回波平面成像（EPI）弛豫测量法来测量局部肿瘤氧张力的新方法。我们现在将使用这种新方法对一组大小匹配的R3327-AT1邓宁前列腺大鼠肿瘤进行的氧张力测量结果与使用传统极谱法（Eppendorf组织氧测定仪）的测量结果进行比较。使用这两种方法发现了相似的氧张力分布，并且两种技术均显示，体积大于3.5 cm³的肿瘤比体积较小的肿瘤（体积小于2 cm³）的氧合程度明显更低（P < 0.0001）。使用（19）F EPI方法，我们还研究了对呼吸刺激的反应。对于一组小肿瘤，将吸入氧浓度从33%提高到100% O₂会使肿瘤氧合显著增加（P < 0.0001）。相比之下，一组大肿瘤的平均pO₂未观察到变化。不考虑肿瘤大小而对单个肿瘤区域进行分析时，发现呼吸100% O₂时观察到的最大pO₂与平均基线pO₂之间存在很强的相关性。这些结果进一步证明了（19）F EPI在评估局部肿瘤氧合变化方面的有用性。
