Kallas E G, Reynolds K, Andrews J, Treanor J J, Evans T G
Infectious Diseases Unit, University of Rochester, NY 14642, USA.
J Interferon Cytokine Res. 1999 Jul;19(7):751-5. doi: 10.1089/107999099313596.
Tumor necrosis factor-alpha (TNF-alpha) is often measured in the serum or plasma of patients with severe infections, and marked elevation correlates with poor outcome. The relationship of TNF-alpha to protection from disease is frequently not observed because prospective studies of infectious agents are difficult to perform. We took advantage of a human antiviral influenza challenge study to correlate TNF-alpha production with seroconversion and symptom development. TNF-alpha production was measured by ELISA in the plasma compartment or was measured by intracellular production at the single cell level in the monocyte gated population. Monocyte TNF-alpha was associated with asymptomatic seroconversion, whereas there was no change in the plasma at the times measured. Measurement of TNF-alpha at the single cell level by flow cytometry may allow for better differentiation of the protective role of this cytokine in future studies.
肿瘤坏死因子-α（TNF-α）通常在重症感染患者的血清或血浆中进行检测，其显著升高与不良预后相关。由于对感染因子进行前瞻性研究难度较大，因此常常无法观察到TNF-α与疾病防护之间的关系。我们利用一项人类抗病毒流感激发试验研究，将TNF-α的产生与血清转化及症状发展相关联。通过酶联免疫吸附测定法（ELISA）检测血浆中TNF-α的产生，或在单核细胞门控群体中通过单细胞水平的细胞内产生情况进行检测。单核细胞TNF-α与无症状血清转化相关，而在所测时间点血浆中未出现变化。通过流式细胞术在单细胞水平检测TNF-α，可能有助于在未来研究中更好地区分这种细胞因子的保护作用。
