Apse M P, Aharon G S, Snedden W A, Blumwald E
Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada.
Science. 1999 Aug 20;285(5431):1256-8. doi: 10.1126/science.285.5431.1256.
Agricultural productivity is severely affected by soil salinity. One possible mechanism by which plants could survive salt stress is to compartmentalize sodium ions away from the cytosol. Overexpression of a vacuolar Na+/H+ antiport from Arabidopsis thaliana in Arabidopsis plants promotes sustained growth and development in soil watered with up to 200 millimolar sodium chloride. This salinity tolerance was correlated with higher-than-normal levels of AtNHX1 transcripts, protein, and vacuolar Na+/H+ (sodium/proton) antiport activity. These results demonstrate the feasibility of engineering salt tolerance in plants.
土壤盐渍化严重影响农业生产力。植物能够在盐胁迫下存活的一种可能机制是将钠离子分隔在胞质溶胶之外。拟南芥液泡Na⁺/H⁺逆向转运蛋白在拟南芥植株中的过表达促进了在浇灌高达200毫摩尔氯化钠的土壤中的持续生长和发育。这种耐盐性与高于正常水平的AtNHX1转录本、蛋白质以及液泡Na⁺/H⁺（钠/质子）逆向转运蛋白活性相关。这些结果证明了在植物中工程化培育耐盐性的可行性。
