Rassidakis G Z, Tani E, Svedmyr E, Porwit A, Skoog L
Laboratory of Histology and Embryology, University of Athens Medical School, Athens, Greece.
Cancer. 1999 Aug 25;87(4):216-23.
Cytologic distinction between follicle center lymphoma (FCL) and mantle cell lymphoma (MCL) is difficult with cytomorphology alone and requires immunophenotyping. The current study describes the distinction between follicle center and mantle cell lymphoma made with fine-needle aspiration (FNA) material.
One hundred ten cases primarily diagnosed and classified on FNA material as centroblastic-centrocytic (CBCC) and centrocytic (CC) non-Hodgkin lymphomas (NHLs) (Kiel classification) were included in the study. An additional retrospective immunocytochemical analysis was performed on frozen cytospin preparations using the monoclonal antibodies Bcl-2, CD10, CD5, CD23, CD43, and immunoglobulin M.
The initial diagnostic workup classified 106 cases as CBCC-NHL and 4 as CC-NHL. The immunophenotype Bcl-2(+), CD10(+/-), CD5(-), CD23(-/+), CD43(-) was observed in 93 of 106 previously reported CBCC NHLs. In 11 of 106 cytospin preparations, neoplastic B cells expressed the CD5 pan T marker and, as a group, showed the pattern Bcl (+/-), CD10(-/+), CD5(+), CD23(-), CD43(+), which is considered typical of MCL. Based on the additional immunocytochemical data, all but 2 of the tumors were reclassified as FCL (n = 93) and MCL (n = 15). The mean proliferation fraction measured by MIB-1 (Ki-67) immunoreactivity was 16.3% and 17.5% in FCL and MCL, respectively. The revised cytopathologic diagnosis correlated significantly (P < 10(-9)) with the histology of 65 patients who underwent surgical excision biopsy.
Subclassification of follicle-derived low grade NHL can be established with high accuracy on FNA material if cytomorphology is corroborated by a complete immunophenotypic analysis, which can be performed on both fresh and frozen stored cytospin material. The currently used criteria can be applied to aspirated cells for a conclusive cytopathologic diagnosis of MCL, which is of great clinical importance. Cancer (Cancer Cytopathol)
仅靠细胞形态学很难对滤泡中心淋巴瘤（FCL）和套细胞淋巴瘤（MCL）进行细胞学区分，需要进行免疫表型分析。本研究描述了利用细针穿刺（FNA）材料对滤泡中心淋巴瘤和套细胞淋巴瘤的区分。
本研究纳入了110例最初根据FNA材料诊断并分类为中心母细胞-中心细胞型（CBCC）和中心细胞型（CC）非霍奇金淋巴瘤（NHL）（ Kiel分类）的病例。使用单克隆抗体Bcl-2、CD10、CD5、CD23、CD43和免疫球蛋白M对冷冻细胞涂片制备物进行了额外的回顾性免疫细胞化学分析。
最初的诊断检查将106例分类为CBCC-NHL，4例分类为CC-NHL。在先前报告的106例CBCC NHL中，93例观察到免疫表型为Bcl-2(+)、CD10(+/-)、CD5(-)、CD23(-/+)、CD43(-)。在106例细胞涂片制备物中的11例中，肿瘤性B细胞表达CD5泛T标志物，并且作为一个群体，显示出Bcl(+/-)、CD10(-/+)、CD5(+)、CD23(-)、CD43(+)的模式，这被认为是MCL的典型模式。根据额外的免疫细胞化学数据，除2例肿瘤外，所有肿瘤均重新分类为FCL（n = 93）和MCL（n = 15）。通过MIB-1（Ki-67）免疫反应性测量的平均增殖分数在FCL和MCL中分别为16.3%和17.5%。修订后的细胞病理学诊断与65例行手术切除活检患者的组织学结果显著相关（P < 10(-9)）。
如果通过完整的免疫表型分析证实细胞形态学，那么在FNA材料上可以高精度地建立滤泡源性低级别NHL的亚分类，完整的免疫表型分析可以在新鲜和冷冻保存的细胞涂片材料上进行。目前使用的标准可应用于吸出细胞，以对MCL进行确定性的细胞病理学诊断，这具有重要的临床意义。癌症（癌症细胞病理学）
