Kang H, Jia L Z, Suh K Y, Tang L, Schuman E M
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Learn Mem. 1996 Sep-Oct;3(2-3):188-96. doi: 10.1101/lm.3.2-3.188.
The neurotrophins are a class of signaling molecules known for their growth and survival-promoting activities during neuronal development. Recent studies suggest that the neurotrophins, including brain-derived neurotrophic factor (BDNF), can also dramatically influence synaptic transmission in the adult hippocampus. The experiments described in this paper indicate that ability of BDNF to potentiate synaptic transmission in the hippocampus relies on functional Trk B receptors. Moreover, the rate at which BDNF is applied to hippocampal synapses is also a potent determinant of whether synaptic potentiation will result. Hippocampal slices perfused with BDNF at a very slow flow rate (e.g., < or = 25 ml/hr) did not show synaptic potentiation. Increasing the rate of BDNF application resulted in synaptic potentiation in which the magnitude and onset kinetics of the potentiation were determined by the rate of BDNF delivery. Immunocytochemical analysis of BDNF detected with confocal microscopy confirmed these electrophysiological observations, indicating that the penetration of BDNF into hippocampal slices is influenced dramatically by the perfusion rate.
神经营养因子是一类信号分子，因其在神经元发育过程中的生长和促存活活性而闻名。最近的研究表明，包括脑源性神经营养因子（BDNF）在内的神经营养因子，也能显著影响成年海马体中的突触传递。本文所述实验表明，BDNF增强海马体突触传递的能力依赖于功能性TrkB受体。此外，将BDNF应用于海马体突触的速率也是突触增强是否会产生的一个重要决定因素。以非常慢的流速（例如，≤25毫升/小时）灌注BDNF的海马体切片未显示突触增强。增加BDNF的应用速率导致突触增强，其中增强的幅度和起始动力学由BDNF递送的速率决定。用共聚焦显微镜检测BDNF的免疫细胞化学分析证实了这些电生理观察结果，表明BDNF进入海马体切片的渗透受到灌注速率的显著影响。
