Ben-Gigirey B, Vieites Baaptista de Sousa J M, Villa T G, Barros-Velazquez J
Centro Técnico Nacional de Conservación de Productos de la Pesca (ANFACO-CECOPESCA), Vigo, Spain.
J Food Prot. 1999 Aug;62(8):933-9. doi: 10.4315/0362-028x-62.8.933.
Two hundred twenty-seven bacterial strains were isolated from fresh and frozen albacore stored either at -18 or -25 degrees C and investigated for their abilities to produce biogenic amines. As a preliminary screening, all 227 strains were tested in either Niven or Niven modified medium, which allowed the selection of 25 presumptive histamine-producing strains. High-pressure liquid chromatography revealed that only 10 of the 25 strains selected were able to produce low histamine concentrations (<25 ppm) in tryptic soy broth medium supplemented with 2% histidine. None of the 25 strains tested produced putrescine or spermine, whereas 6 strains produced spermidine. Histamine production by Stenotrophomonas maltophilia strain 25MC6 was not prevented at 4 degrees C, and the levels of this amine reached concentrations of 25.8 ppm after 6 days. Three S. maltophilia strains showed strong lysine-decarboxylating activity. Their cadaverine formation capacity was determined by high-pressure liquid chromatography in tryptic soy broth supplemented with 1% lysine; this revealed that the three S. maltophilia strains tested produced more than 700 ppm of cadaverine during the first 24 h of incubation at 37 degrees C. S. maltophilia strain 15MF, initially obtained from fresh albacore tuna, produced up to 2,399 ppm and 4,820 ppm of cadaverine after 24 and 48 h of incubation at 37 degrees C, respectively. To our knowledge, this is the first report on histamine and cadaverine production by strains of the species S. maltophilia, previously known as Pseudomonas and Xanthomonas maltophilia, isolated from fresh and frozen albacore tuna.
从储存在-18℃或-25℃的新鲜和冷冻长鳍金枪鱼中分离出227株细菌菌株，并对它们产生生物胺的能力进行了研究。作为初步筛选，所有227株菌株都在尼文或改良尼文培养基中进行了测试，从中筛选出25株疑似产组胺菌株。高压液相色谱分析表明，在添加2%组氨酸的胰蛋白胨大豆肉汤培养基中，所选的25株菌株中只有10株能够产生低组胺浓度（<25 ppm）。测试的25株菌株均未产生腐胺或精胺，而有6株产生亚精胺。嗜麦芽窄食单胞菌菌株25MC6在4℃时仍能产生组胺，6天后该胺的浓度达到25.8 ppm。三株嗜麦芽窄食单胞菌菌株表现出较强的赖氨酸脱羧酶活性。通过高压液相色谱法在添加1%赖氨酸的胰蛋白胨大豆肉汤中测定它们生成尸胺的能力；结果表明，在37℃孵育的最初24小时内，测试的三株嗜麦芽窄食单胞菌菌株产生的尸胺超过700 ppm。最初从新鲜长鳍金枪鱼中分离得到的嗜麦芽窄食单胞菌菌株15MF，在37℃分别孵育24小时和48小时后，产生的尸胺分别高达2399 ppm和4820 ppm。据我们所知，这是首次报道从新鲜和冷冻长鳍金枪鱼中分离出的嗜麦芽窄食单胞菌（以前称为嗜麦芽假单胞菌和嗜麦芽黄单胞菌）菌株产生组胺和尸胺的情况。
