表皮葡萄球菌纤维蛋白原结合蛋白的功能研究
Functional studies of a fibrinogen binding protein from Staphylococcus epidermidis.
作者信息
Pei L, Palma M, Nilsson M, Guss B, Flock J I
机构信息
Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Karolinska Institutet, Huddinge University Hospital, F82, S-141 86 Huddinge, Sweden.
出版信息
Infect Immun. 1999 Sep;67(9):4525-30. doi: 10.1128/IAI.67.9.4525-4530.1999.
Abstract
A gene encoding a fibrinogen binding protein from Staphylococcus epidermidis was previously cloned, and the nucleotide sequence was determined. A portion of the gene encompassing the fibrinogen binding domain has now been subcloned in an expression-fusion vector. The fusion protein can bind to fibrinogen in a capture enzyme-linked immunosorbent assay and can be purified by fibrinogen affinity chromatography. This protein can completely inhibit the adherence of S. epidermidis to immobilized fibrinogen, suggesting that the adherence of S. epidermidis to fibrinogen is mainly due to this protein. Antibodies against this fibrinogen binding protein were also found to efficiently block the adherence of S. epidermidis to immobilized fibrinogen. Despite homology with clumping factors A and B from S. aureus (cell surface-associated proteins binding to fibrinogen), binding involved the beta chain of fibrinogen rather than the gamma chain, as in clumping factor A.
摘要
先前已克隆出编码表皮葡萄球菌纤维蛋白原结合蛋白的基因，并测定了其核苷酸序列。现在，包含纤维蛋白原结合结构域的部分基因已亚克隆到表达融合载体中。在捕获酶联免疫吸附测定中，融合蛋白可与纤维蛋白原结合，并可通过纤维蛋白原亲和层析进行纯化。该蛋白可完全抑制表皮葡萄球菌对固定化纤维蛋白原的黏附，这表明表皮葡萄球菌对纤维蛋白原的黏附主要归因于该蛋白。还发现针对这种纤维蛋白原结合蛋白的抗体可有效阻断表皮葡萄球菌对固定化纤维蛋白原的黏附。尽管与金黄色葡萄球菌的聚集因子A和B（与纤维蛋白原结合的细胞表面相关蛋白）具有同源性，但与聚集因子A一样，结合涉及纤维蛋白原的β链而非γ链。
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