铜绿假单胞菌孔蛋白可诱导源自大鼠精囊的上皮细胞系发生凋亡。
Porin from Pseudomonas aeruginosa induces apoptosis in an epithelial cell line derived from rat seminal vesicles.
作者信息
Buommino E, Morelli F, Metafora S, Rossano F, Perfetto B, Baroni A, Tufano M A
机构信息
CNR International Institute of Genetics and Biophysics, Area di Ricerca del C.N.R., Medical School, 2nd University of Naples, Italy.
出版信息
Infect Immun. 1999 Sep;67(9):4794-800. doi: 10.1128/IAI.67.9.4794-4800.1999.
Abstract
Micromolar concentrations of porin, purified from the outer membranes of Pseudomonas aeruginosa, induced in vitro the classic morphological and biochemical signs of apoptosis in an epithelial cell line (SVC1) derived from the rat seminal vesicle secretory epithelium. The programmed cell death (PCD) was p53 independent and associated with significant decrease of bcl-2 expression, a marked increase of c-myc transcriptional activity, and an absence of the mRNA coding for tissue transglutaminase. The Ca(2+) influx, caused by the porin treatment of SVC1 cells, appears to play an important role in the triggering of apoptosis in our biological model. The possibility that the porin property of inducing PCD plays a role in the infertility of individuals chronically infected by gram-negative bacteria is discussed.
摘要
从铜绿假单胞菌外膜纯化得到的微摩尔浓度的孔蛋白，在体外诱导了源自大鼠精囊分泌上皮的上皮细胞系（SVC1）出现经典的凋亡形态学和生化特征。程序性细胞死亡（PCD）不依赖p53，与bcl-2表达显著降低、c-myc转录活性显著增加以及组织转谷氨酰胺酶编码mRNA缺失有关。孔蛋白处理SVC1细胞所引起的Ca(2+)内流，在我们的生物学模型中似乎在触发凋亡方面起重要作用。文中讨论了孔蛋白诱导PCD的特性在革兰氏阴性菌慢性感染个体不育中发挥作用的可能性。
相似文献
1
Porin from Pseudomonas aeruginosa induces apoptosis in an epithelial cell line derived from rat seminal vesicles.铜绿假单胞菌孔蛋白可诱导源自大鼠精囊的上皮细胞系发生凋亡。
Infect Immun. 1999 Sep;67(9):4794-800. doi: 10.1128/IAI.67.9.4794-4800.1999.
2
Sodium butyrate/retinoic acid costimulation induces apoptosis-independent growth arrest and cell differentiation in normal and ras-transformed seminal vesicle epithelial cells unresponsive to retinoic acid.丁酸钠/视黄酸共刺激可诱导对视黄酸无反应的正常和ras转化精囊上皮细胞发生不依赖凋亡的生长停滞和细胞分化。
J Mol Endocrinol. 2000 Feb;24(1):83-94. doi: 10.1677/jme.0.0240083.
3
Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in .外膜通透性：抗生素和各种营养物质绕过孔蛋白。
Proc Natl Acad Sci U S A. 2021 Aug 3;118(31). doi: 10.1073/pnas.2107644118.
4
Targeting of the pro-apoptotic VDAC-like porin (PorB) of Neisseria gonorrhoeae to mitochondria of infected cells.淋病奈瑟菌促凋亡的类电压依赖性阴离子通道孔蛋白（PorB）定位于被感染细胞的线粒体。
EMBO J. 2000 Oct 16;19(20):5332-43. doi: 10.1093/emboj/19.20.5332.
5
Whole-Cell-Based Assay To Evaluate Structure Permeation Relationships for Carbapenem Passage through the Pseudomonas aeruginosa Porin OprD.基于全细胞的分析方法，用于评估碳青霉烯类药物通过铜绿假单胞菌孔蛋白OprD的结构渗透关系。
ACS Infect Dis. 2017 Apr 14;3(4):310-319. doi: 10.1021/acsinfecdis.6b00197. Epub 2017 Feb 17.
6
Neisserial porin (PorB) causes rapid calcium influx in target cells and induces apoptosis by the activation of cysteine proteases.奈瑟菌孔蛋白（PorB）可导致靶细胞中钙离子快速内流，并通过激活半胱氨酸蛋白酶诱导细胞凋亡。
EMBO J. 1999 Jan 15;18(2):339-52. doi: 10.1093/emboj/18.2.339.
7
The pilus and porin of Neisseria gonorrhoeae cooperatively induce Ca(2+) transients in infected epithelial cells.淋病奈瑟菌的菌毛和孔蛋白协同诱导感染上皮细胞中的Ca(2+)瞬变。
Cell Microbiol. 2005 Dec;7(12):1736-48. doi: 10.1111/j.1462-5822.2005.00586.x.
8
Pseudomonas aeruginosa outer membrane protein F is an adhesin in bacterial binding to lung epithelial cells in culture.铜绿假单胞菌外膜蛋白F是细菌在培养中与肺上皮细胞结合的一种黏附素。
Microb Pathog. 2002 Sep;33(3):109-14. doi: 10.1006/mpat.2002.0514.
9
Bacterial porins stimulate bone resorption.细菌孔蛋白刺激骨吸收。
Infect Immun. 1997 Apr;65(4):1313-6. doi: 10.1128/iai.65.4.1313-1316.1997.
10
Involvement of CPP32/Caspase-3 in c-Myc-induced apoptosis.CPP32/半胱天冬酶-3参与c-Myc诱导的细胞凋亡。
Oncogene. 1998 Jan 22;16(3):387-98. doi: 10.1038/sj.onc.1201779.
引用本文的文献
1
Cytotoxins: Mechanisms of Cytotoxicity and Impact on Inflammatory Responses.细胞毒素：细胞毒性的机制及其对炎症反应的影响。
Cells. 2023 Jan 3;12(1):195. doi: 10.3390/cells12010195.
2
Bacteriospermia and Male Infertility: Role of Oxidative Stress.菌精症与男性不育：氧化应激的作用。
Adv Exp Med Biol. 2022;1358:141-163. doi: 10.1007/978-3-030-89340-8_7.
3
Bacterial Infections Affect Male Fertility: A Focus on the Oxidative Stress-Autophagy Axis.细菌感染影响男性生育能力：聚焦氧化应激-自噬轴
Front Cell Dev Biol. 2021 Oct 21;9:727812. doi: 10.3389/fcell.2021.727812. eCollection 2021.
4
Outer membrane vesicles from Neisseria gonorrhoeae target PorB to mitochondria and induce apoptosis.淋病奈瑟菌外膜囊泡靶向 PorB 至线粒体并诱导细胞凋亡。
PLoS Pathog. 2018 Mar 30;14(3):e1006945. doi: 10.1371/journal.ppat.1006945. eCollection 2018 Mar.
5
Vibrio cholerae Porin OmpU Induces Caspase-independent Programmed Cell Death upon Translocation to the Host Cell Mitochondria.霍乱弧菌孔蛋白OmpU易位至宿主细胞线粒体后可诱导非半胱天冬酶依赖性程序性细胞死亡。
J Biol Chem. 2015 Dec 25;290(52):31051-68. doi: 10.1074/jbc.M115.670182. Epub 2015 Nov 11.
6
Platelet-activating factor receptor initiates contact of Acinetobacter baumannii expressing phosphorylcholine with host cells.血小板激活因子受体启动表达磷酰胆碱的鲍曼不动杆菌与宿主细胞的接触。
J Biol Chem. 2012 Aug 3;287(32):26901-10. doi: 10.1074/jbc.M112.344556. Epub 2012 Jun 11.
7
Evaluation of the protective effect of bovine lactoferrin against lipopolysaccharides in a bovine mammary epithelial cell line.评价牛乳铁蛋白对牛乳腺上皮细胞系脂多糖的保护作用。
Vet Res Commun. 2010 Mar;34(3):267-76. doi: 10.1007/s11259-010-9351-z. Epub 2010 Mar 30.
8
Bacterial ortholog of mammalian translocator protein (TSPO) with virulence regulating activity.具有毒力调节活性的哺乳动物转位蛋白（TSPO）的细菌直系同源物。
PLoS One. 2009 Jun 30;4(6):e6096. doi: 10.1371/journal.pone.0006096.
9
Reactive nitrogen intermediates and monokines induce caspase-3 mediated macrophage apoptosis by anaerobically stressed Salmonella typhi.活性氮中间体和单核因子通过厌氧应激的伤寒沙门氏菌诱导caspase-3介导的巨噬细胞凋亡。
Clin Exp Immunol. 2007 Nov;150(2):368-74. doi: 10.1111/j.1365-2249.2007.03503.x. Epub 2007 Sep 20.
10
Expression of Pseudomonas aeruginosa toxin ExoS effectively induces apoptosis in host cells.铜绿假单胞菌毒素ExoS的表达可有效诱导宿主细胞凋亡。
Infect Immun. 2006 Dec;74(12):6557-70. doi: 10.1128/IAI.00591-06. Epub 2006 Sep 11.
本文引用的文献
1
The mitochondrion as an integrator/coordinator of cell death pathways.线粒体作为细胞死亡途径的整合者/协调者。
Cell Death Differ. 1998 Jun;5(6):547. doi: 10.1038/sj.cdd.4400387.
2
Neisserial porin (PorB) causes rapid calcium influx in target cells and induces apoptosis by the activation of cysteine proteases.奈瑟菌孔蛋白（PorB）可导致靶细胞中钙离子快速内流，并通过激活半胱氨酸蛋白酶诱导细胞凋亡。
EMBO J. 1999 Jan 15;18(2):339-52. doi: 10.1093/emboj/18.2.339.
3
Apoptotic cell death in response to LPS.对脂多糖的凋亡性细胞死亡
Prog Clin Biol Res. 1998;397:235-42.
4
Lack of induction of tissue transglutaminase but activation of the preexisting enzyme in c-Myc-induced apoptosis of CHO cells.在c-Myc诱导的CHO细胞凋亡中，组织转谷氨酰胺酶未被诱导，但已存在的酶被激活。
Biochem Biophys Res Commun. 1997 Jul 18;236(2):280-4. doi: 10.1006/bbrc.1997.6969.
5
Porins of Pseudomonas aeruginosa induce release of tumor necrosis factor alpha and interleukin-6 by human leukocytes.铜绿假单胞菌的孔蛋白可诱导人白细胞释放肿瘤坏死因子α和白细胞介素-6。
Infect Immun. 1997 May;65(5):1683-7. doi: 10.1128/iai.65.5.1683-1687.1997.
6
Bacterial porins stimulate bone resorption.细菌孔蛋白刺激骨吸收。
Infect Immun. 1997 Apr;65(4):1313-6. doi: 10.1128/iai.65.4.1313-1316.1997.
7
Signal transduction in the mammalian cell during bacterial attachment and entry.细菌附着和进入过程中哺乳动物细胞内的信号转导。
Cell. 1993 Jun 4;73(5):903-20. doi: 10.1016/0092-8674(93)90270-z.
8
Thymocyte apoptosis induced by p53-dependent and independent pathways.由p53依赖和非依赖途径诱导的胸腺细胞凋亡。
Nature. 1993 Apr 29;362(6423):849-52. doi: 10.1038/362849a0.
9
ras oncogene-induced transformation of a rat seminal vesicle epithelial cell line produces a marked increase of adenylate cyclase and protein kinase C activities.Ras癌基因诱导大鼠精囊上皮细胞系转化，导致腺苷酸环化酶和蛋白激酶C活性显著增加。
FEBS Lett. 1993 Sep 27;331(1-2):150-4. doi: 10.1016/0014-5793(93)80315-l.
10
Outer-membrane porins from gram-negative bacteria stimulate platelet-activating-factor biosynthesis by cultured human endothelial cells.革兰氏阴性菌的外膜孔蛋白可刺激培养的人内皮细胞合成血小板活化因子。
Eur J Biochem. 1993 Jun 15;214(3):685-93. doi: 10.1111/j.1432-1033.1993.tb17969.x.
Zotero 插件