• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

布鲁氏菌属的外膜蛋白Omp10、Omp16和Omp19是脂蛋白。

Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins.

作者信息

Tibor A, Decelle B, Letesson J J

机构信息

Laboratoire de Microbiologie et d'Immunologie, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium.

出版信息

Infect Immun. 1999 Sep;67(9):4960-2. doi: 10.1128/IAI.67.9.4960-4962.1999.

DOI:10.1128/IAI.67.9.4960-4962.1999
PMID:10456959
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC96837/
Abstract

The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane proteins of Brucella spp. contain a potential bacterial lipoprotein processing sequence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.

摘要

布鲁氏菌属的外膜蛋白Omp10、Omp16和Omp19的推导序列含有潜在的细菌脂蛋白加工序列。用Triton X-114提取后，这三种蛋白质分配到去污剂相中。三种蛋白质的加工受到球蛋白霉素（一种脂蛋白信号肽酶的特异性抑制剂）的抑制。用单克隆抗体从[³H]棕榈酸标记的流产布鲁氏菌裂解物中对这三种蛋白质进行放射免疫沉淀。这些结果表明Omp10、Omp16和Omp19是脂蛋白。

相似文献

1
Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins.布鲁氏菌属的外膜蛋白Omp10、Omp16和Omp19是脂蛋白。
Infect Immun. 1999 Sep;67(9):4960-2. doi: 10.1128/IAI.67.9.4960-4962.1999.
2
Molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of Brucella abortus.流产布鲁氏菌两种次要外膜蛋白在感染绵羊和牛体内的分子特征、存在情况及免疫原性
Infect Immun. 1996 Jan;64(1):100-7. doi: 10.1128/iai.64.1.100-107.1996.
3
Effect of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice.omp10或omp19缺失对牛布鲁氏菌外膜特性及小鼠毒力的影响。
Infect Immun. 2002 Oct;70(10):5540-6. doi: 10.1128/IAI.70.10.5540-5546.2002.
4
Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection.用重组布鲁氏菌属外膜蛋白Omp16或Omp19与佐剂一起免疫可诱导特异性CD4 +和CD8 + T细胞，以及对流产布鲁氏菌感染的全身和口腔保护。
Infect Immun. 2009 Jan;77(1):436-45. doi: 10.1128/IAI.01151-08. Epub 2008 Nov 3.
5
Molecular cloning, nucleotide sequence, and occurrence of a 16.5-kilodalton outer membrane protein of Brucella abortus with similarity to pal lipoproteins.流产布鲁氏菌16.5千道尔顿外膜蛋白的分子克隆、核苷酸序列及与pal脂蛋白的相似性研究
Infect Immun. 1994 Sep;62(9):3633-9. doi: 10.1128/iai.62.9.3633-3639.1994.
6
Brucella ovis PA mutants for outer membrane proteins Omp10, Omp19, SP41, and BepC are not altered in their virulence and outer membrane properties.绵羊种布鲁氏菌外膜蛋白Omp10、Omp19、SP41和BepC的PA突变体在毒力和外膜特性方面未发生改变。
Vet Microbiol. 2016 Apr 15;186:59-66. doi: 10.1016/j.vetmic.2016.02.010. Epub 2016 Feb 20.
7
Omp19 Enables to Evade the Antimicrobial Activity From Host's Proteolytic Defense System.Omp19 使 能够逃避宿主蛋白酶防御系统的抗菌活性。
Front Immunol. 2019 Jun 26;10:1436. doi: 10.3389/fimmu.2019.01436. eCollection 2019.
8
Survey of Omp19 immunogenicity against Brucella abortus and Brucella melitensis: influence of nanoparticulation versus traditional immunization.针对流产布鲁氏菌和羊种布鲁氏菌的Omp19免疫原性研究：纳米颗粒化与传统免疫的影响
Vet Res Commun. 2015 Dec;39(4):217-28. doi: 10.1007/s11259-015-9645-2. Epub 2015 Sep 22.
9
Lipoproteins, not lipopolysaccharide, are the key mediators of the proinflammatory response elicited by heat-killed Brucella abortus.脂蛋白而非脂多糖，是热灭活布鲁氏菌流产株引发促炎反应的关键介质。
J Immunol. 2004 Oct 1;173(7):4635-42. doi: 10.4049/jimmunol.173.7.4635.
10
The protein moiety of Brucella abortus outer membrane protein 16 is a new bacterial pathogen-associated molecular pattern that activates dendritic cells in vivo, induces a Th1 immune response, and is a promising self-adjuvanting vaccine against systemic and oral acquired brucellosis.布鲁氏菌 abortus 外膜蛋白 16 的蛋白部分是一种新的细菌病原体相关分子模式，可在体内激活树突状细胞，诱导 Th1 免疫应答，是一种有前途的自身佐剂疫苗，可预防全身和口服布鲁氏菌病。
J Immunol. 2010 May 1;184(9):5200-12. doi: 10.4049/jimmunol.0902209. Epub 2010 Mar 29.

引用本文的文献

1
Brucellosis: Bacteriology, pathogenesis, epidemiology and role of the metallophores in virulence: a review.布鲁氏菌病：细菌学、发病机制、流行病学及金属离子载体在毒力中的作用：综述
Front Cell Infect Microbiol. 2025 Jul 8;15:1621230. doi: 10.3389/fcimb.2025.1621230. eCollection 2025.
2
Beyond survival to domination: 's multilayered strategies for evading host immune responses.从生存到主宰：[病原体名称]逃避宿主免疫反应的多重策略 。（你提供的原文中“Beyond survival to domination: 's multilayered strategies for evading host immune responses.”这里's前面应该有具体指代的病原体之类的词汇，我按照格式要求翻译了，但你可根据实际情况补充完整）
Front Microbiol. 2025 Jun 18;16:1608617. doi: 10.3389/fmicb.2025.1608617. eCollection 2025.
3
A Thermosensitive and Degradable Chitin-Based Hydrogel as a Brucellosis Vaccine Adjuvant.一种基于几丁质的热敏可降解水凝胶作为布鲁氏菌病疫苗佐剂
Polymers (Basel). 2024 Oct 4;16(19):2815. doi: 10.3390/polym16192815.
4
Design of a multi-epitope vaccine against brucellosis fused to IgG-fc by an immunoinformatics approach.通过免疫信息学方法设计一种与IgG-fc融合的抗布鲁氏菌病多表位疫苗。
Front Vet Sci. 2023 Oct 23;10:1238634. doi: 10.3389/fvets.2023.1238634. eCollection 2023.
5
Brucellosis detection and the role of spp. cell wall proteins.布鲁氏菌病检测及 spp. 细胞壁蛋白的作用。
Vet World. 2023;16(7):1390-1399. doi: 10.14202/vetworld.2023.1390-1399. Epub 2023 Jul 4.
6
Molecular Survey of Field Isolates using Sequence-Based PCR of Outer Membrane Protein 31.基于外膜蛋白 31 的序列 PCR 对现场分离株进行分子调查。
Arch Razi Inst. 2022 Jun 1;77(3):1261-1267. doi: 10.22092/ARI.2021.355107.1664. eCollection 2022 Jun.
7
Proteomic and bioinformatic analyses of proteins in the outer membrane and extracellular compartments and outer membrane vesicles of Liberibacter species.对韧皮部杆菌属物种外膜、细胞外区室及外膜囊泡中的蛋白质进行蛋白质组学和生物信息学分析。
Front Microbiol. 2022 Sep 26;13:977710. doi: 10.3389/fmicb.2022.977710. eCollection 2022.
8
A disordered region retains the full protease inhibitor activity and the capacity to induce CD8 T cells of the oral vaccine adjuvant U-Omp19.一个无序区域保留了口服疫苗佐剂U-Omp19的全部蛋白酶抑制剂活性以及诱导CD8 T细胞的能力。
Comput Struct Biotechnol J. 2022 Sep 6;20:5098-5114. doi: 10.1016/j.csbj.2022.08.054. eCollection 2022.
9
Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis.主要外膜蛋白联合应用于布鲁氏菌病血清学诊断的评估
Infect Drug Resist. 2022 Jul 28;15:4093-4100. doi: 10.2147/IDR.S372411. eCollection 2022.
10
Registered Influenza Viral Vector Based Vaccine for Cattle in Kazakhstan: Age-Wise Safety and Efficacy Studies.哈萨克斯坦基于注册流感病毒载体的牛用疫苗：按年龄分组的安全性和有效性研究。
Front Cell Infect Microbiol. 2021 Jul 1;11:669196. doi: 10.3389/fcimb.2021.669196. eCollection 2021.

本文引用的文献

1
Brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family Rhizobiaceae.布鲁氏菌外膜脂蛋白与根瘤菌科细菌具有共同的抗原决定簇。
Clin Diagn Lab Immunol. 1999 Jul;6(4):627-9. doi: 10.1128/CDLI.6.4.627-629.1999.
2
A novel outer membrane lipoprotein, LolB (HemM), involved in the LolA (p20)-dependent localization of lipoproteins to the outer membrane of Escherichia coli.一种新型外膜脂蛋白LolB（HemM），参与脂蛋白在LolA（p20）依赖下定位到大肠杆菌外膜的过程。
EMBO J. 1997 Dec 1;16(23):6947-55. doi: 10.1093/emboj/16.23.6947.
3
Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay.在间接酶联免疫吸附测定中，布鲁氏菌感染的牛、绵羊和山羊对八种纯化的重组布鲁氏菌蛋白的体液免疫反应。
Clin Diagn Lab Immunol. 1997 Sep;4(5):556-64. doi: 10.1128/cdli.4.5.556-564.1997.
4
Cloning and nucleotide sequence analysis of a Brucella abortus gene encoding an 18 kDa immunoreactive protein.
Microb Pathog. 1997 Apr;22(4):241-6. doi: 10.1006/mpat.1996.0108.
5
Characterization, occurrence, and molecular cloning of a 39-kilodalton Brucella abortus cytoplasmic protein immunodominant in cattle.牛体内占免疫主导地位的39千道尔顿布鲁氏菌流产亚种胞质蛋白的特性、存在情况及分子克隆
Infect Immun. 1997 Feb;65(2):495-502. doi: 10.1128/iai.65.2.495-502.1997.
6
Cloning, nucleotide sequence, and expression of the Brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein.编码免疫原性主要外膜蛋白的布鲁氏菌羊种omp31基因的克隆、核苷酸序列及表达
Infect Immun. 1996 Sep;64(9):3744-51. doi: 10.1128/iai.64.9.3744-3751.1996.
7
Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene.绵羊布鲁氏菌主要25千道尔顿外膜蛋白编码基因的核苷酸序列及表达：与其他布鲁氏菌属物种相比，因该基因缺失导致抗原漂移的证据。
Infect Immun. 1996 Jun;64(6):2047-55. doi: 10.1128/iai.64.6.2047-2055.1996.
8
Molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of Brucella abortus.流产布鲁氏菌两种次要外膜蛋白在感染绵羊和牛体内的分子特征、存在情况及免疫原性
Infect Immun. 1996 Jan;64(1):100-7. doi: 10.1128/iai.64.1.100-107.1996.
9
Molecular cloning, nucleotide sequence, and occurrence of a 16.5-kilodalton outer membrane protein of Brucella abortus with similarity to pal lipoproteins.流产布鲁氏菌16.5千道尔顿外膜蛋白的分子克隆、核苷酸序列及与pal脂蛋白的相似性研究
Infect Immun. 1994 Sep;62(9):3633-9. doi: 10.1128/iai.62.9.3633-3639.1994.
10
Cloning and nucleotide sequence of the gene coding for the major 25-kilodalton outer membrane protein of Brucella abortus.流产布鲁氏菌主要25千道尔顿外膜蛋白编码基因的克隆与核苷酸序列分析
J Bacteriol. 1995 Apr;177(7):1911-4. doi: 10.1128/jb.177.7.1911-1914.1995.