Tibor Anne, Wansard Valérie, Bielartz Valery, Delrue Rose-May, Danese Isabelle, Michel Patrick, Walravens Karl, Godfroid Jacques, Letesson Jean-Jacques
Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, B-5000 Namur, Belgium.
Infect Immun. 2002 Oct;70(10):5540-6. doi: 10.1128/IAI.70.10.5540-5546.2002.
The distinctive properties of Brucella outer membrane have been considered to be critical for Brucella sp. virulence. Among the outer membrane molecules possibly related to these properties, Omp10 and Omp19 are immunoreactive outer membrane lipoproteins. Moreover, these proteins of Brucella could constitute a new family of outer membrane proteins specifically encountered in the family RHIZOBIACEAE: We evaluated the impact of omp10 or omp19 deletion on Brucella abortus outer membrane properties and virulence in mice. The omp10 mutant was dramatically attenuated for survival in mice and was defective for growth in minimal medium but was not impaired in intracellular growth in vitro, nor does it display clear modification of the outer membrane properties. Significantly fewer brucellae were recovered from the spleens of mice infected with the omp19 mutant than from those of mice infected with the parent strain at 4 and 8 weeks postinfection. The omp19 mutant exhibited an increase in sensitivity to the polycation polymyxin B and to sodium deoxycholate. These results indicate that inactivation of the omp19 gene alters the outer membrane properties of B. abortus.
布鲁氏菌外膜的独特性质被认为对布鲁氏菌属的毒力至关重要。在可能与这些性质相关的外膜分子中，Omp10和Omp19是具有免疫反应性的外膜脂蛋白。此外，布鲁氏菌的这些蛋白质可能构成了根瘤菌科中特有的一个新的外膜蛋白家族：我们评估了omp10或omp19缺失对布鲁氏菌流产亚种外膜性质和小鼠毒力的影响。omp10突变体在小鼠体内的存活能力显著减弱，在基本培养基中生长存在缺陷，但在体外细胞内生长未受损害，其外膜性质也未显示出明显改变。在感染后4周和8周，从感染omp19突变体的小鼠脾脏中回收的布鲁氏菌明显少于感染亲本菌株的小鼠。omp19突变体对聚阳离子多粘菌素B和脱氧胆酸钠的敏感性增加。这些结果表明，omp19基因的失活改变了流产布鲁氏菌的外膜性质。
