Peagler F D, Redman R S
Oral Pathology Research Laboratory, Department of Veterans Affairs Medical Center, Washington, District of Columbia 20422, USA.
Anat Rec. 1999 Sep 1;256(1):72-7. doi: 10.1002/(SICI)1097-0185(19990901)256:1<72::AID-AR9>3.0.CO;2-D.
Information on ductal differentiation in the developing rat parotid gland is sparse. Striated and excretory ducts are rich in a number of enzymes related to ion movement. The objective of this investigation was to delineate histochemically the chronology of two of these, ouabain-sensitive Na(+),K(+)-ATPase and NADH-DE, in the developing rat parotid gland. Parotid glands were excised from rats at representative ages from 20 days in utero to 42 days. Enzyme histochemistry was performed on air-dried frozen sections. For Na(+), K(+)-ATPase, some sections also were fixed in phosphate-buffered formalin. Ouabain blocked Na(+),K(+)-ATPase activity, and neither enzyme reacted without substrate. Weak Na(+),K(+)-ATPase reactions were initially seen in unfixed sections at 1 day, and increased steadily to the adult pattern of strong (concentrated basolaterally) in striated ducts and excretory ducts, respectively, and weak to modest (diffuse) in acini and intercalated ducts at 28 days. In fixed sections, localization was sharper but the reaction was somewhat reduced. NADH-DE was modest in terminal buds and ducts before birth, then progressively changed to the adult pattern of weak in acini and intercalated ducts and strong (concentrated basally and luminally) in striated and excretory ducts at 28 days. As demonstrated by enzyme histochemistry of Na(+),K(+)-ATPase and NADH-DE, differentiation of rat parotid striated ducts and excretory ducts occurs mainly between birth and 28 days. Anat Rec 256:72-77, 1999. Published 1999 Wiley-Liss, Inc.
关于发育中大鼠腮腺导管分化的信息很少。纹状管和排泄管富含多种与离子转运相关的酶。本研究的目的是通过组织化学方法描绘发育中大鼠腮腺中其中两种酶，即哇巴因敏感的钠钾ATP酶和NADH - 脱氢酶的变化过程。从子宫内20天到42天的不同代表性年龄的大鼠身上切除腮腺。对空气干燥的冰冻切片进行酶组织化学检测。对于钠钾ATP酶，一些切片还用磷酸盐缓冲福尔马林固定。哇巴因可阻断钠钾ATP酶的活性，且两种酶在无底物时均无反应。在未固定的切片中，1天时最初可见微弱的钠钾ATP酶反应，到28天时，分别在纹状管和排泄管中稳定增加至成年模式的强反应（集中在基底外侧），在腺泡和闰管中为弱至中等反应（弥漫性）。在固定切片中，定位更清晰，但反应有所减弱。出生前，NADH - 脱氢酶在终末芽和导管中反应中等，然后逐渐转变为成年模式，28天时在腺泡和闰管中为弱反应，在纹状管和排泄管中为强反应（集中在基底和管腔）。如钠钾ATP酶和NADH - 脱氢酶的酶组织化学所示，大鼠腮腺纹状管和排泄管的分化主要发生在出生至28天之间。《解剖学记录》256:72 - 77，1999年。1999年由威利 - 利斯公司出版。
