Seo Il-Young, Kim Miwon, Lee Jongun, Ryu Sun-Youl
Department of Oral and Maxillofacial Surgery, Chonnam National University, Professional Graduate School of Dentistry, Gwangju, Korea.
Department of Nursing, Chonnam National University College of Nursing, Gwangju, Korea.
Electrolyte Blood Press. 2008 Jun;6(1):9-14. doi: 10.5049/EBP.2008.6.1.9. Epub 2008 Jun 30.
A role of nitric oxide (NO) in the regulation of sodium transporters and water channels in the salivary gland was investigated. Male Sprague-Dawley rats were treated with N(G) -nitro-L-arginine methyl ester (L-NAME, 100 mg/L drinking water) for 1 week. The control group was supplied with normal tap water. The expression of Na(+),K(+)-ATPase, type 2 Na(+)/K(+)/2Cl(-) cotransporter (NKCC2), type 1 Na(+)/H(+) exchanger (NHE1), α-subunit of epithelial sodium transporter (ENaC), and aquaporin-5 (AQP5) and aquaporin-1 (AQP1) proteins were determined in the submandibular gland by Western blot analysis. Following the treatment with L-NAME, the expression of Na(+),K(+)-ATPase α1-subunit, NKCC2, NHE1, and ENaC α-subunit increased significantly. On the contrary, the expression of AQP5 was significantly decreased, while that of AQP1 was not significantly altered. These findings indicate that the sodium transporters and water channels may be under a tonic regulatory influence of NO in the salivary gland.
研究了一氧化氮（NO）在唾液腺钠转运体和水通道调节中的作用。将雄性Sprague-Dawley大鼠用N（G）-硝基-L-精氨酸甲酯（L-NAME，100mg/L饮用水）处理1周。对照组供应正常自来水。通过蛋白质免疫印迹分析测定下颌下腺中Na（+），K（+）-ATP酶、2型Na（+）/K（+）/2Cl（-）共转运体（NKCC2）、1型Na（+）/H（+）交换体（NHE1）、上皮钠转运体（ENaC）的α亚基以及水通道蛋白5（AQP5）和水通道蛋白1（AQP1）的表达。用L-NAME处理后，Na（+），K（+）-ATP酶α1亚基、NKCC2、NHE1和ENaCα亚基的表达显著增加。相反，AQP5的表达显著降低，而AQP1的表达没有显著改变。这些发现表明，钠转运体和水通道可能受到唾液腺中NO的紧张性调节影响。
