某些乙二胺四乙酸和环己烷-1,2-二胺四乙酸络合物在柱色谱和离子电动色谱中的分离选择性

Separation selectivity of some ethylenediaminetetraacetic acid and cyclohexane-1,2-diaminetetraacetic acid complexes in column and ion electrokinetic chromatography.

作者信息

Krokhin O V, Adamov A V, Hoshino H, Shpigun O A, Yotsuyanagi T

机构信息

Chemistry Department, Lomonosov Moscow State University, Russia.

出版信息

J Chromatogr A. 1999 Jul 30;850(1-2):269-76. doi: 10.1016/s0021-9673(99)00371-4.

DOI:10.1016/s0021-9673(99)00371-4

PMID:10457487

Abstract

The complexes of Mn2+, Cd2+, Fe3+, Pb2+, Ni2+, Co2+, Zn2+ and Cu2+ with EDTA and cyclohexane-1,2-diaminetetraacetic acid (CDTA) were separated and detected in column and ion electrokinetic chromatography with suppressed conductivity and direct UV detection, respectively. In column ion chromatography (IC) these complexes were separated on an IonPac AS4A anion-exchange column (Dionex, USA). Parameters of carrier electrolyte, which were examined in the ion electrokinetic chromatography (IEKC) mode, include polymer and sulfate concentrations. In IEKC separation selectivity of complexes with poly(diallyldimethylammonium) cation as modifier is similar as for an IonPac AS4A column both for EDTA and CDTA chelates. It was shown that the ion-exchange capacity of the electrokinetic system is more than 100-times lower than the capacity of the IC column for the same peak resolution. In comparison with column main advantages of electrokinetic version are high separation efficiency (220,000-390,000 theoretical plates) and the absence of the analyte interaction with the sorbent matrix.

摘要

分别在抑制电导柱离子色谱和直接紫外检测的离子电动色谱中，分离并检测了Mn2+、Cd2+、Fe3+、Pb2+、Ni2+、Co2+、Zn2+和Cu2+与乙二胺四乙酸（EDTA）和环己烷-1,2-二胺四乙酸（CDTA）形成的配合物。在柱离子色谱（IC）中，这些配合物在IonPac AS4A阴离子交换柱（美国戴安公司）上进行分离。在离子电动色谱（IEKC）模式下考察的载体电解质参数包括聚合物和硫酸盐浓度。在IEKC中，以聚（二烯丙基二甲基铵）阳离子为改性剂时，配合物的分离选择性对于EDTA和CDTA螯合物来说与IonPac AS4A柱类似。结果表明，在相同的峰分辨率下，电动系统的离子交换容量比IC柱的容量低100倍以上。与柱色谱相比，电动色谱的主要优点是分离效率高（220,000 - 390,000理论塔板数）以及分析物与吸附剂基质无相互作用。