Kharchenko O V, Cernjuk V N, Butovich I A
Institute of Bioorganic Chemistry and Petrolchemistry, National Academy of Sciences of Ukraine, Kyiv.
Ukr Biokhim Zh (1999). 1999 Jan-Feb;71(1):33-7.
Linoleic acid oxidation by 12-lipoxygenase from porcine leukocytes has been studied as affected by linoleyl-hydroxamic acid. Linoleyl-hydroxamic acid has been found to be an effective inhibitor of porcine leucocyte 12-lipoxygenase. Aerobic preincubation of 12-lipoxygenase with 0.1-6 microM of linoleyl-hydroxamic acid led to a time- and dose-dependent inhibition of the enzyme. The inhibitor's concentration able to induce a 50% loss of the enzyme activity with and without 15-min preincubation were 3.5 and 0.65 microM, respectively. Experimental results obeyed a kinetic scheme, which supposed 2 extra substrate molecules bounding with the enzyme-substrate complex in the presence of linoleyl-hydroxamic acid.
研究了亚油基异羟肟酸对猪白细胞12-脂氧合酶催化亚油酸氧化的影响。已发现亚油基异羟肟酸是猪白细胞12-脂氧合酶的有效抑制剂。12-脂氧合酶与0.1 - 6微摩尔亚油基异羟肟酸进行需氧预孵育会导致该酶出现时间和剂量依赖性抑制。在有和没有15分钟预孵育的情况下，能够导致酶活性丧失50%的抑制剂浓度分别为3.5微摩尔和0.65微摩尔。实验结果符合一种动力学方案，该方案假定在亚油基异羟肟酸存在下有2个额外的底物分子与酶 - 底物复合物结合。
