Papapanagiotou A, Zachari A, Tzivras M, Koufali M M, Kalofoutis A
Department of Biological Chemistry, University of Athens, School of Medicine, Greece.
In Vivo. 1999 May-Jun;13(3):259-62.
Liver phospholipid concentrations were determined in rats after the administration of diazepam (5 mg/Kg/day), for a period of two months. Increased concentrations of total phospholipids (P < 0.05), phosphatidylcholine (P < 0.05) and phosphatidylinositol (P < 0.05) were found in the rats taking diazepam. In contrast, a decreased concentration of phosphatidylserine (P < 0.01) was observed in the same group of animals. In addition, changes in the concentration of rat liver mitochondrial phospholipids after the administration of diazepam during the same period of time were determined. Increased concentrations of total phospholipids (P < 0.01), phosphatidylcholine (P < 0.001) and diphosphatidylglycerol (P < 0.001) were found in the rats treated with diazepam. In contrast, decreased phosphatidylserine (P < 0.001) and phosphatidylinositol (P < 0.01) concentrations were observed in the same group of animals. The considerable changes observed in liver phospholipids and individual classes of liver mitochondrial phospholipids induced by long-term administration of diazepam, possibly suggest a stimulation of liver phospholipid biosynthesis. This effect may be related to enzymatic systems which are involved in phospholipid pathways, and are linked to benzodiazepinergic binding sites.
在给予大鼠地西泮（5毫克/千克/天）两个月后，测定其肝脏磷脂浓度。服用地西泮的大鼠中，总磷脂（P < 0.05）、磷脂酰胆碱（P < 0.05）和磷脂酰肌醇（P < 0.05）的浓度升高。相比之下，在同一组动物中观察到磷脂酰丝氨酸浓度降低（P < 0.01）。此外，还测定了在同一时间段给予地西泮后大鼠肝脏线粒体磷脂浓度的变化。接受地西泮治疗的大鼠中，总磷脂（P < 0.01）、磷脂酰胆碱（P < 0.001）和二磷脂酰甘油（P < 0.001）的浓度升高。相比之下，在同一组动物中观察到磷脂酰丝氨酸（P < 0.001）和磷脂酰肌醇（P < 0.01）浓度降低。长期给予地西泮后，肝脏磷脂和肝脏线粒体磷脂各类别中观察到的显著变化，可能表明肝脏磷脂生物合成受到刺激。这种作用可能与参与磷脂途径并与苯二氮䓬能结合位点相关的酶系统有关。
