从不同地理区域分离的卡他莫拉菌中，脉冲场凝胶电泳产生的DNA限制性酶切图谱。
DNA restriction patterns produced by pulsed-field gel electrophoresis in Moraxella catarrhalis isolated from different geographical areas.
作者信息
Martinez G, Ahmed K, Zheng C H, Watanabe K, Oishi K, Nagatake T
机构信息
Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Japan.
出版信息
Epidemiol Infect. 1999 Jun;122(3):417-22. doi: 10.1017/s0950268899002381.
Pulsed field gel electrophoresis (PFGE) of the genomic DNA of Moraxella catarrhalis was done in 172 strains isolated from sputum of patients with respiratory infections in Nagasaki (130 strains), Europe (14 strains), Thailand (6 strains), Uganda (3 strains), Bangladesh (5 strains) and Kuwait (14 strains). Restriction endonuclease with SmaI generated 4-16 DNA fragments ranging from 1000 kb to 24.25 kb and was classified into 31 major groups. It was found that there were wide variations of DNA restriction patterns of strains isolated from the same and different geographical areas. DNA restriction patterns of strains isolated in Nagasaki during the last 12 years showed dynamic changes of the predominant strains in each time period. We conclude from this study that PFGE is a suitable method to document interstrain variation in M. catarrhalis.
对从长崎（130株）、欧洲（14株）、泰国（6株）、乌干达（3株）、孟加拉国（5株）和科威特（14株）呼吸道感染患者痰液中分离出的172株卡他莫拉菌的基因组DNA进行了脉冲场凝胶电泳（PFGE）分析。用SmaI限制性内切酶产生了4 - 16个DNA片段，大小从1000 kb到24.25 kb不等，并分为31个主要组。结果发现，从同一地理区域和不同地理区域分离出的菌株的DNA限制性图谱存在广泛差异。过去12年长崎分离出的菌株的DNA限制性图谱显示了每个时间段主要菌株的动态变化。我们从这项研究得出结论，PFGE是记录卡他莫拉菌菌株间变异的合适方法。
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