比较用于卡他莫拉菌脉冲场凝胶电泳分型的限制酶。
Comparison of restriction enzymes for pulsed-field gel electrophoresis typing of Moraxella catarrhalis.
机构信息
Microbiology Department, Hospital Universitari Bellvitge, IDIBELL, University of Barcelona, Barcelona, Spain.
出版信息
J Clin Microbiol. 2013 Jul;51(7):2448-52. doi: 10.1128/JCM.00823-13. Epub 2013 May 15.
Abstract
NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI.
摘要
NotI 是用于对卡他莫拉菌进行分型的最常见的限制酶，但它无法消化呼吸道样本中的基因组 DNA。改进的脉冲场凝胶电泳 (PFGE) 方法确定 SpeI 是对该细菌进行分型的最佳选择，它对临床样本有很好的限制作用，与 NotI 的聚类相关性也很好。
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