类风湿关节炎患者多个关节中积累的T细胞克隆型的特征分析。
Characterisation of T cell clonotypes that accumulated in multiple joints of patients with rheumatoid arthritis.
作者信息
Kurokawa M, Kato T, Masuko-Hongo K, Ueda S, Kobata T, Okubo M, Nishimaki T, Akaza T, Yoshino S, Kasukawa R, Nishioka K, Yamamoto K
机构信息
Rheumatology, Immunology and Genetic Program, Institute of Medical Science, St Marianna University School of Medicine, Kawasaki, Japan.
出版信息
Ann Rheum Dis. 1999 Sep;58(9):546-53. doi: 10.1136/ard.58.9.546.
OBJECTIVE
To investigate whether identical T cell clonotypes accumulate in multiple rheumatoid joints, the clonality of T cells that had infiltrated into synovial tissue (ST) samples simultaneously obtained from multiple joints of patients with rheumatoid arthritis (RA) was analysed.
METHODS
T cell receptor (TCR) beta gene transcripts, amplified by reverse transcription-polymerase chain reaction from ST and peripheral blood lymphocytes of five RA patients, were subjected to single strand conformation polymorphism analysis and DNA sequencing.
RESULTS
Approximately 40% of accumulated T cell clonotypes found in one joint of a patient were found in multiple joints in the same patient. Furthermore, identical amino acid sequences were found in TCR beta junctional regions of these clonotypes from different patients with at least one HLA molecule match.
CONCLUSIONS
The T cell clonotypes accumulating in multiple rheumatoid joints may be involved in the perpetuation of polyarthritis by reacting to antigens common to these multiple joints.
目的
为了研究相同的T细胞克隆型是否会在多个类风湿关节中积累，我们分析了从类风湿关节炎（RA）患者多个关节同时获取的滑膜组织（ST）样本中浸润的T细胞的克隆性。
方法
通过逆转录聚合酶链反应从5例RA患者的ST和外周血淋巴细胞中扩增T细胞受体（TCR）β基因转录本，进行单链构象多态性分析和DNA测序。
结果
在患者一个关节中发现的约40%的积累T细胞克隆型在同一患者的多个关节中也被发现。此外，在不同患者的这些克隆型的TCRβ连接区发现了相同的氨基酸序列，且至少有一个HLA分子匹配。
结论
在多个类风湿关节中积累的T细胞克隆型可能通过对这些多个关节共有的抗原产生反应而参与多关节炎的持续存在。
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