Lee J H, Griffiths W J, Zantout I, Welsh J D
Am J Dig Dis. 1978 Dec;23(12):1129-31. doi: 10.1007/BF01072890.
Intestinal mucosa from 40 patients obtained by fiber-endoscopic biopsy was assayed for disaccharidases to determine suitability of this tissue for assay. The combined specimens from each patient provided 4.7-38.7 mg of tissue, adequate in all instances for duplicate determinations of protein, lactase, sucrase, and maltase. Tissue remained for assays of palatinase in 39 instances, trehalase and cellobiase in 37, and alkaline phosphatase in 22 cases. Twenty-four subjects had normal lactose tolerance tests and normal sucrase/lactase ratios. Thirteen patients with abnormal oral lactose tolerance tests were identified as having a primary low lactase activity on the basis of elevated sucrase/lactase ratios. This ratio was most helpful in making the diagnosis of a primary low lactase, since the mucosal specimens were not obtained from comparable areas. Tissue from three subjects with an abnormally low maltase was unsuitable for diagnosis. Endoscopic biopsy of mucosa appears to be satisfactory for disaccharidase assays in most instances.
通过纤维内镜活检获取了40例患者的肠黏膜，对其双糖酶进行检测，以确定该组织用于检测的适用性。每位患者的合并标本提供了4.7 - 38.7毫克组织，在所有情况下都足以重复测定蛋白质、乳糖酶、蔗糖酶和麦芽糖酶。在39例中留有组织用于检测腭酶，37例用于检测海藻糖酶和纤维二糖酶，22例用于检测碱性磷酸酶。24名受试者乳糖耐量试验正常，蔗糖酶/乳糖酶比值正常。13例口服乳糖耐量试验异常的患者，根据蔗糖酶/乳糖酶比值升高，被确定为原发性乳糖酶活性低下。该比值在诊断原发性低乳糖酶方面最有帮助，因为黏膜标本并非取自可比区域。3例麦芽糖酶异常低的受试者的组织不适合用于诊断。在大多数情况下，内镜下黏膜活检对于双糖酶检测似乎是令人满意的。
