Rothstein E P, Anderson E L, Decker M D, Poland G A, Reisinger K S, Blatter M M, Jacobson R M, Mink C A, Gennevois D, Izu A E, Sinangil F, Langenberg A G
Pennridge Pediatric Associates, Sellersville, PA 18960, USA.
Vaccine. 1999 Aug 6;17(23-24):2999-3006. doi: 10.1016/s0264-410x(99)00164-4.
Recent data indicate that Bordetella pertussis can be an important cause of illness in adolescents and adults. In a randomized observer- and subject-blinded study, adults (> or = 18 years of age) received an acellular pertussis (aP) vaccine containing genetically inactivated pertussis toxin (PT), filamentous hemagglutinin (FHA) and pertactin (PRN), or a saline placebo, and were monitored for safety and immunogenicity. IgG antibodies to PT, FHA, and PRN were measured by enzyme-linked immunosorbent assay (ELISA) and PT neutralization by a Chinese hamster ovary (CHO) cell assay. Local reactions, more common in the aP group, were mild and transient. One month after immunization, geometric mean ELISA antibody concentrations for the aP and placebo groups, respectively, were: anti-PT, 463 and 7.6; anti-FHA, 417 and 18; and anti-PRN, 855 and 14. The anti-PT neutralization titers for the aP and placebo groups were 1:3439 and 1:58 respectively. This aP vaccine is a safe and immunogenic candidate booster vaccine against pertussis for adults.
近期数据表明，百日咳博德特氏菌可能是青少年和成人患病的一个重要原因。在一项随机、观察者和受试者双盲研究中，成年人（≥18岁）接种了一种含基因灭活百日咳毒素（PT）、丝状血凝素（FHA）和百日咳黏附素（PRN）的无细胞百日咳（aP）疫苗，或生理盐水安慰剂，并对安全性和免疫原性进行监测。通过酶联免疫吸附测定（ELISA）检测针对PT、FHA和PRN的IgG抗体，并通过中国仓鼠卵巢（CHO）细胞测定法检测PT中和情况。局部反应在aP组更为常见，症状轻微且短暂。免疫后1个月，aP组和安慰剂组的ELISA抗体几何平均浓度分别为：抗PT，463和7.6；抗FHA，417和18；抗PRN，855和14。aP组和安慰剂组的抗PT中和效价分别为1:3439和1:58。这种aP疫苗是一种安全且具有免疫原性的成人百日咳候选加强疫苗。
