百日咳血清学：IgG 抗-PT ELISA 替代 CHO 细胞检测法的评估。
Pertussis serology: assessment of IgG anti-PT ELISA for replacement of the CHO cell assay.
机构信息
Department of Microbiological Surveillance and Research, Statens Serum Institut, Copenhagen, Denmark.
出版信息
APMIS. 2010 Dec;118(12):968-72. doi: 10.1111/j.1600-0463.2010.02664.x. Epub 2010 Sep 2.
Two types of serological assays are commonly used for the assessment of pertussis vaccine-induced antibodies; the Chinese hamster ovary cell (CHO cell) assay and the immunoglobulin G (IgG) anti pertussis toxin (PT) enzyme-linked immunosorbent assay (IgG anti-PT ELISA). Recently, both the techniques have been modified to improve performance with sera with interfering activity (CHO cell assay) or with heat-treated sera (IgG anti-PT ELISA). These two improved techniques were compared by the analysis of 100 individual serum samples from a previous clinical trial and 213 sera from a longitudinal serum collection from 20 Danish adults recently vaccinated with the Danish acellular pertussis vaccine. The comparison showed a significant linear correlation between the results of the two assays with a p-value of <0.0001 for the 100 individual samples. We, therefore, conclude that the improved IgG anti-PT ELISA can be used as a replacement for the often troublesome and time-consuming CHO cell assay for the measurement of vaccine-induced human antibodies to PT.
两种血清学检测方法常用于评估百日咳疫苗诱导的抗体
中国仓鼠卵巢细胞（CHO 细胞）检测法和免疫球蛋白 G（IgG）抗百日咳毒素（PT）酶联免疫吸附试验（IgG 抗-PT ELISA）。最近，这两种技术都进行了改进，以提高具有干扰活性的血清（CHO 细胞检测法）或热处理血清（IgG 抗-PT ELISA）的性能。通过对先前临床试验的 100 份个体血清样本和最近接种丹麦无细胞百日咳疫苗的 213 份丹麦成年人纵向血清采集的分析，比较了这两种改进的技术。结果表明，两种检测方法的结果具有显著的线性相关性，100 份个体样本的 p 值<0.0001。因此，我们得出结论，改进的 IgG 抗-PT ELISA 可替代通常麻烦且耗时的 CHO 细胞检测法，用于测量疫苗诱导的人体针对 PT 的抗体。
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