da Silva O S, Grunewald J
Institute of Tropical Medicine, University of Tuebingen, Germany.
Mem Inst Oswaldo Cruz. 1999 Sep-Oct;94(5):579-82. doi: 10.1590/s0074-02761999000500002.
American cutaneous leishmaniasis (ACL) is endemic in the State of Rio Grande do Sul, Brazil. As reports of nearby hospitals suggest, the Parque Estadual do Turvo seems to serve as a source for the disease. During three months from November 1996 to February 1997 we collected, in this park, 2,228 sandflies (10 Lutzomyia species and 2 6species). We applied the polymerase chain reaction to 920 females which belong to the following species: Lutzomyia migonei, Lu. pessoai, Lu. fischeri, Lu. misionensis, Lu. lanei, Lu. neivai, Lu. shannoni, and Lu. monticola, in an attempt to verify natural infection by Leishmania (Viannia), the causative agent of ACL. Le. (Viannia) infections were demonstrated by DNA amplification from two Lu. pessoai and one Lu. misionensis female. Lu. pessoai have been found with leptomonas in the gut believed to be Le. (V.) braziliensis in other endemic areas of northeastern and southeastern Brazil. However, Lu. misionensis has never been found carrying a natural infection of Le. (Viannia).
美洲皮肤利什曼病（ACL）在巴西南里奥格兰德州呈地方性流行。附近医院的报告显示，图尔沃州立公园似乎是该病的一个源头。1996年11月至1997年2月的三个月间，我们在这个公园里采集了2228只白蛉（10种卢氏白蛉属和2种其他属）。我们对属于以下种类的920只雌性白蛉应用了聚合酶链反应：米氏卢氏白蛉、佩氏卢氏白蛉、费氏卢氏白蛉、米西奥内斯卢氏白蛉、拉内氏卢氏白蛉、内瓦伊卢氏白蛉、香农氏卢氏白蛉和蒙蒂科拉卢氏白蛉，试图验证ACL病原体利什曼原虫（维阿尼亚亚属）的自然感染情况。通过对两只佩氏卢氏白蛉和一只米西奥内斯卢氏白蛉雌性进行DNA扩增，证实了利什曼原虫（维阿尼亚亚属）感染。在巴西东北部和东南部的其他流行地区，已在佩氏卢氏白蛉的肠道中发现了被认为是巴西利什曼原虫（维阿尼亚亚属）的细滴虫。然而，从未发现米西奥内斯卢氏白蛉携带利什曼原虫（维阿尼亚亚属）的自然感染。
