Luz E, Membrive N, Castro E A, Dereure J, Pratlong F, Dedet J A, Pandey A, Thomaz-Soccol V
Departamento de Patologia Básica, Universidade Federal do Paraná, Centro Politécnico Jardim das Américas, Curitiba, Brazil.
Ann Trop Med Parasitol. 2000 Sep;94(6):623-31. doi: 10.1080/00034983.2000.11813585.
The phlebotomine sandflies in the northern areas of the state of Paraná, Brazil, particularly those in the '16a' health region, were investigated over a 3-year period. Using CDC light traps (with and without hamster bait) and Shannon traps (with lights and horse or human bait), 16 species were collected from seven municipal districts which were known foci for cutaneous leishmaniasis: Arapongas; Apucarana; Cambira; Marumbi; Faxinal; Florestópolis; and Sabáudia. Although the frequency at which each species was collected varied with the collection site, Lutzomyia whitmani predominated (62.0% of all the sandflies collected), followed by Lu. fischeri (13.3%), Lu. pessoai (10.8%), Lu. migonei (8.2%) and Lu. intermedia (2.8%). Lutzomyia monticola, Lu. shanonni, Lu. firmatoi, Lu. lanei, Lu. alphabetica, Lu. misionensis, Lu. correalimai, Lu. cortellezzii, Lu. longipenis, Brumptomyia brumpti and B. nitzulescui together represented the remaining 3.0% of the collected sandflies. Three of the 1961 female sandflies collected and dissected in the municipal district of Cambira, where a recent case of cutaneous leishmaniasis had been registered, were found to have flagellates in their guts. All three were Lu. whitmani. The parasites from each of these infections were successfully isolated in NNN and 'Tobie and Evans' media and/or by inoculation into a hind foot of a golden hamster. The results of isoenzyme electrophoresis indicated that all three isolates were of Leishmania (Viannia) braziliensis.
在三年时间里，对巴西巴拉那州北部地区，特别是“16a”健康区域的白蛉进行了调查。使用疾控中心诱蚊灯（有仓鼠诱饵和无仓鼠诱饵）和香农诱捕器（有灯光和马或人诱饵），从七个已知为皮肤利什曼病疫源地的市辖区采集到了16种白蛉：阿拉蓬加斯；阿普卡拉纳；坎比拉；马伦比；法西纳尔；弗洛雷斯托波利斯；以及萨博迪亚。尽管每个物种的采集频率因采集地点而异，但惠特曼白蛉占主导地位（占所有采集到的白蛉的62.0%），其次是费氏白蛉（13.3%）、佩索阿白蛉（10.8%）、米戈内伊白蛉（8.2%）和中间白蛉（2.8%）。蒙蒂科拉白蛉、香农白蛉、菲尔马托伊白蛉、拉内伊白蛉、字母白蛉、米西奥内斯白蛉、科雷亚利马白蛉、科尔泰莱齐白蛉、长阴茎白蛉、布氏白蛉和尼祖莱斯基白蛉总共占采集到的白蛉的其余3.0%。在坎比拉市辖区（该地最近登记了一例皮肤利什曼病病例）采集并解剖的1961只雌性白蛉中，有三只在其肠道中发现有鞭毛虫。这三只都是惠特曼白蛉。这些感染中的每一种寄生虫都成功地在NNN和“托比和埃文斯”培养基中分离出来和/或通过接种到金黄仓鼠的后足中分离出来。同工酶电泳结果表明，所有三个分离株均为巴西利什曼原虫（维安尼亚亚种）。
