Sawada S, Kokeguchi S, Nishimura F, Takashiba S, Murayama Y
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
Microbios. 1999;98(391):133-40.
The nearly complete 16S rRNA gene sequences for oral Gram-negative anaerobic motile bacteria, Centipeda periodontii, Selenomonas sputigena and Selenomonas species (formerly S. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. To determine the phylogenetic characterization of these bacteria, their 16S rRNA gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. The 16S rRNA gene sequences of these bacteria were similar to those of Selenomonas ruminantium and Schwartzia succinivorans isolated from rumens, and to Pectinatus cerevisiiphilus isolated from spoiled beer. Among oral bacteria, the nucleotide sequence analysis of these bacteria revealed high nucleotide similarity to Veillonella species, whereas low similarity to oral motile bacteria such as Campylobacter species. Phylogenetic analysis clearly confirmed that C. periodontii and two Selenomonas species were classified as relatives of a group besides Selenomonas, Schwartzia, and Pectinatus species, and not as close relatives to oral motile bacteria, such as Campylobacter species. These results suggest that such oral Gram-negative anaerobic motile bacteria are close relatives of oral bacteria.
为了揭示口腔革兰氏阴性厌氧运动细菌、牙周蜈蚣菌、生痰月形单胞菌和月形单胞菌属（以前的生痰月形单胞菌模式菌株）与其他口腔运动细菌的关系，测定了它们近乎完整的16S rRNA基因序列。为了确定这些细菌的系统发育特征，获取了它们的16S rRNA基因序列，并与先前报道的核糖体序列数据库中的序列进行比较。这些细菌的16S rRNA基因序列与从瘤胃中分离出的反刍月形单胞菌和琥珀酸嗜黑菌，以及从变质啤酒中分离出的啤酒果胶杆菌的序列相似。在口腔细菌中，对这些细菌的核苷酸序列分析显示与韦荣氏菌属具有高度的核苷酸相似性，而与弯曲菌属等口腔运动细菌的相似性较低。系统发育分析清楚地证实，牙周蜈蚣菌和两种月形单胞菌被归类为除月形单胞菌、嗜黑菌和果胶杆菌属之外的一组亲缘关系，而不是与弯曲菌属等口腔运动细菌的近亲。这些结果表明，这类口腔革兰氏阴性厌氧运动细菌是口腔细菌的近亲。
