Schleifer K H, Leuteritz M, Weiss N, Ludwig W, Kirchhof G, Seidel-Rüfer H
Lehrstuhl für Mikrobiologie, Technische Universität München, Federal Republic of Germany.
Int J Syst Bacteriol. 1990 Jan;40(1):19-27. doi: 10.1099/00207713-40-1-19.
A collection of 47 strains of obligately anaerobic, gram-negative, rod-shaped bacteria that were isolated mainly from spoiled beer and pitching yeast was studied to learn more about their taxonomic positions. A new species of the genus Pectinatus, Pectinatus frisingensis, a new species of the genus Selenomonas, Selenomonas lacticifex, and a new genus comprising two species, Zymophilus raffinosivorans and Zymophilus paucivorans, are described. All of the strains contained directly cross-linked meso-diaminopimelic acid-containing peptidoglycan and in addition the diamine cadaverine or (rarely) putrescine. The diamine was covalently linked to the alpha-carboxyl group of D-glutamic acid in the peptide subunit of peptidoglycan. Lipid F was also found as a characteristic cellular compound. The phylogenetic relationships of members of these new species were examined by reverse transcriptase sequencing of 16S rRNA or by DNA-DNA hybridization studies or both. All of the organisms belong to the subdivision containing species with gram-negative cell walls within the phylum of gram-positive bacteria. This finding is in good agreement with the presence of a peptidoglycan that contains diamine.
对主要从变质啤酒和接种酵母中分离出的47株专性厌氧、革兰氏阴性、杆状细菌进行了研究，以进一步了解它们的分类地位。描述了果胶杆菌属的一个新物种，弗里斯果胶杆菌（Pectinatus frisingensis），栖乳酸新月形单胞菌（Selenomonas lacticifex），新月形单胞菌属的一个新物种，以及包含两个物种的一个新属，嗜棉子糖发酵单胞菌（Zymophilus raffinosivorans）和寡食发酵单胞菌（Zymophilus paucivorans）。所有菌株均含有直接交联的含内消旋二氨基庚二酸的肽聚糖，此外还含有二胺尸胺或（很少）腐胺。二胺与肽聚糖肽亚基中D-谷氨酸的α-羧基共价连接。脂质F也被发现是一种特征性细胞化合物。通过16S rRNA的逆转录酶测序或DNA-DNA杂交研究或两者来检测这些新物种成员的系统发育关系。所有这些生物都属于革兰氏阳性菌门中含有革兰氏阴性细胞壁物种的亚类。这一发现与含有二胺的肽聚糖的存在非常吻合。
